RNAi沉默ERK1-2基因對胃癌SGC7901細胞株Ezrin表達及侵襲遷移能力的影響.pdf

1、南昌大學碩士學位論文RNAi沉默ERK12基因對胃癌SGC7901細胞株Ezrin表達及侵襲遷移能力的影響姓名：涂軼申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：梅金紅201206AbstractABSTRACTobjective：ToinvestigaterelationshipbetweentheRNAisilencingofERKl／2andexpressionofEzringeneinhumangastriccanceri

2、nvitroMethods：TheexpressionofEzrinin80specimensofgastriccarcinoma80specimensofnormalgastrictissuesweredetectedbyimmunohistochemistrymethodConventionalcultureofgastriccancercellsSGC7901，twosiRNAstargetingERK1andERK2respec

3、tivelyweredesignedandsynthesizedaccordingtoitsmRNA，andtheircorrespondingexpressionvectorswereconstructedandtransfectedintoSGC7901cellwithLipofectamineⅣ2000TheinhibitionofERKlandERK2expression，togetherwiththeexpressionofE

4、zrinwereanalyzedbyRTPCR，theproteinlevelofthosegenesweredetectedbywesternblotanalysisTranswellmethodwasappliedtomeasurecellinvasionandmigrationResults：Innormalgastrictissuesandgastriccarcinoma，thepercentageofEzrinpositive

5、expressionwas2375％and8875％，respectivelyThedifferencehasastatifticalsignificance(PO05)Comparedwiththecontrols，thegroupsofERKlRNAiandERK2RNAieffectivelyinhibitedtheexpressionofERK1andERK2，withinhibitoryrateof6037％and618％，a

6、mongthetwogroups，theexpressionofEzrinweredownregulatedby6262％and5137％respectivelywithsignificientlydecreasedcellinvasionandmigration(PO05)Conclusion：Comparedwithnormalgastrictissues，Ezrinwasoverexpressioningastriccarcino

7、matissuesTheexpressionofERK1andERK2inSGC7901cellscouldbesupressedbyvetorbasedRNAinterferenceefficientlyTheknockdownofERKl／2causedchangesinEzringeneexpressionrelatedwithcellinvasionandmigration，finialyresultedindecreasedc