秦艽中龍膽苦苷的提取及分離純化工藝研究.pdf

1、南昌大學碩士學位論文秦艽中龍膽苦苷的提取及分離純化工藝研究姓名：郝曉霞申請學位級別：碩士專業(yè)：物理化學指導教師：余淑嫻20071201AbstractABSTRACTExtraction、separation、purificationofgentiopicrosidefromGentiauamacropHyllaPallproducedinGanSuhavebeenstudiedinthispapern圮qualitativeandqu

2、antitativeanalysismethodwasestablishedbyHPLC、UVwitllnormalGPSascomparisonn磚method啪offerfavorableevidencetoanalyzeGPSinthecOILrseofExtractionandseparationn峙experimentswereinaccordance、ⅣimthenationalstandardGB／T500932003、G

3、B／T500962003、GB／T500942003、GB／T5009102003inmeasuringgentianaoriginalherbmoisture、fat、ash、crudefibercontenttheresultsshowedtheaveragelevelsfortargetsaboveasfollowed：1141％，9284％，4094％1234％Parallelexperimentsinvariousdeviat

4、ionsweresmall，themeasurementresultswererelativelycrediblethis伽regardasfightmedicinegeneralchemicalcompositionofgenfianaOnthebasisofthesinglefactor，usinggentiopicrosideastheindex，theeffectsofethanolemounLextractiontime，ex

5、tractiondegreeandextractiontemperatureonextractiontechnologywereevaluatedbyorthogonaldesignmethodandUVn地superiortechnologyofgentiopicrosidewasasfollowed：theethanolwas30ml；extractiontimeof20rainthenumberofextraction4thand

6、theteraperatureWas50。CT1leeffectsofwateramountextractiontimeandextractiondegreeandextractiontemperatureonextractiontechnologyweTeevaluatedbyorthogonaldesignmethodandUltrasonicWaveThesuperiortechnologyofgentiopierosidewas

7、asfollowed：theethanolWas15ml；extractiontimeof40minthenumberofextraction2nd，andthetemperatureWas40。COptimizedSoxhletethanolmethod，usinggentiopicrosideastheindextheoptimumconditionisthesolventdosageof180ml；extractiontimefo

8、r25hUsingcoldimmersionmethod，byorthogonaldesigntheoptimalextractionconditionWasconfirmedThesuperiortechnologyofgentiopicrosideWasasfollowed：ethanolconcentrationof60％，extractiontimeof12handfeedratioofl：9。2extractiontimes1