蕨麻多糖對(duì)吞噬細(xì)胞功能及免疫功能低下小鼠的免疫調(diào)節(jié)作用研究.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文蕨麻多糖對(duì)吞噬細(xì)胞功能及免疫功能低下小鼠的免疫調(diào)節(jié)作用研究姓名：崔東安申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：預(yù)防獸醫(yī)指導(dǎo)教師：陳炅然20080601AbstractPotentillaanserineLisakindofplantwhichbelongstosacraePotentillaandisatypicalstoloniferousclonalherbageintibetanplateau，andpotentilla

2、anserinaListheroottuberofPotentillaanserineLusedforTibetanmedicinalherbPotentillaanserineLpolysaccharide(PAP)isoneofitsmainlyactivecomponentsfromPotentillaanserineLandhaveimmunoregulationeffects，SOPotentillaanserineLispo

3、pularlyknowninfolkmedicineforavarietyofpharmacologicaleffects，suchassatisfyone’Sthirst，invigoratingthespleenandbenefitingthestomachandtonifyingQiandnourishingbloodItWasshownthatcrudeextractsfromPotentillaanserineLhadthee

4、ffectsonexhibitingimmunomodulatoryantiinflammatoryandantibacterialactivities，whileithasbeensuggestedthat，amongtheactiveprinciplesthewatersolublepolysaccharidesmaygreatlycontributetothebiologicalactivityofmanyPotentillaan

5、serineLextractsThestudyWascarriedouttoinvestigatetheimmunomodulatoryeffectsofPAPThemiceperitonealmacrophageswerecollected，thephagocytoticfunctionofmiceperitonealmacrophagesWasmeasured、析tllneutralredabsorptiontestandtheNO

6、releasingbyusingGriessreagent；NeutrophilicleukocyteWascollected，Reactiveoxygenspecies(ROS)stimulatedbyopsonlzedzymosan(OZ)，wasdetectedbyflowcytometry，andthentheactivityofmyelopemxidase(MPO)measured；theimmunosuppressivemi

7、cetreated、析tllCybyintraperitonealinjectiontoinvestigatetheimmunomodulatoryeffectsforPAPonthemice塒tllPAP(50，100，200and400mg／kg)orPAP(50，100，200and400mg／kg)together誦mCyclophosphamide(Cy100mg／kg)Thephagocytotiefunctionofmon

8、onuclearphagocyticsystemwasdetectedwithcarbonclearupmethods，thelevelsofIL6andINF吖inserumweredetected、ⅣitllELISA，meanwhile，theactivityoflysozymeinserumandtheactivityoftheactivityofSOD，GPx，CATinspleentissueweremeasuredTher

9、esultswereasfollow：(1)PAPWasabletopotentializephagocytoticfunctionforperitonealmacrophageinaconcentrationdependentmannerespeciallyintheconcentrationofPAP100Jtg／rnl(PO01)invitro(2)PAPhadpromotedsiginificantlytheactivities

10、ofMPOinadosedependentmanner，especiallyintheconcentrationofPAP200，400“酌m(PO01)invitro(3)PAPcouldincreasingtheROSproductionofneutrophilactivedbyOpsonizedZymosan(Oz)underCyinducedimmunosuppressionbyadjustingsiginificantlyth

11、eintensityofneutrophilrespiratoryburst(4)PAPcouldsiginificantlypotentializeproductionofnitricoxide(N0)invitroinaconcentrationdependentmannerespeciallyintheconcetrationforPAP200，400肛咖l(P001)invitro(5)PAPcouldupgratesignif