HBV DNA卵細(xì)胞載體的構(gòu)建與乙肝患者卵巢組織中HBV DNA的檢測.pdf

1、Objective To construct HBV DNA oocyte vector and to detect if there is HBV DNA in the hepatitis B patients' ovaries? Methods Molecular biology technique was used to extract mice oocytes, single-cell nested poly

2、merase chain reaction(PCR) was used to detect HBV DNA in oocytes after transfection with PBR322-HBV DNA. Fluorescence in situ hybridization was used to detect HBV DNA in hepatits B patients' ovary paraffin sections.

3、 Results ①200 mice oocytes were extracted, of which 180 oocytes were transfected with PBR322-HBV DNA and 20 oocytes weren't transfected. The detection rate of HBV DNA in the mice oocytes after transfection was 69.4％

4、 (125/180) , while 0(0/20) in the oocytes without transfection. And the detection rate of HBV DNA in the washing solution of the transfected oocytes was 10％(3/30). The detection rate of HBV DNA in the transfected mice oo

5、cytes was higher than the oocytes without transfection(P＜0.01), and the washing solution of the transfected oocytes (P＜0.01). There was no statistical significance comparing the detection rate of HBV DNA in the mice oocy

6、tes without transfection to the washing solution of the transfected oocytes (P＞0.05);②The detection rate of HBV DNA of hepatits B patients' ovary paraffin sections was 70％(14/20), the detection rate of HBV DNA of human o

7、vary paraffin sections with HBsAg(-) was 0(0/20). It was statistically significant comparing the detection rate of HBV DNA of hepatits B patients' ovary paraffin sections to that with HBsAg(-)(P＜0.01). Conclusion ①

8、Cation liposome can be a kind of good vector to tranfect oocytes with HBV DNA, and oocytes can be the vector of HBV DNA; ②HBV DNA can be found in the hepatits B patients' ovary paraffin sections with HBsAg(+), suggesting