油茶籽殼活性物質的提取及抗氧化、抑制脂肪酸合酶機理初探.pdf

1、浙江大學生物系統(tǒng)工程與食品科學學院碩士學位論文油茶籽殼活性物質的提取及抗氧化、抑制脂肪酸合酶機理初探姓名：姜天甲申請學位級別：碩士專業(yè)：食品科學指導教師：沈建福20070501浙江大學碩士學位論文ABSTRA(jrABSTRAeTChinaisthelargestcamelliaoilproductioncountryCamelliaoleiferaabelisaspecialoilspeciewhichhasahistoryof230

2、0yearsinourcountryItisestimatedthattherea∞366700hm2Camelliaoleiferaabelwhichproduce1500，000，000kgcamelliaoileveryyearbutalsoleaveUS10，000000，000kgcamelliashellatthes∞etimeSoifwecanmakegooduseofitwemaygetmuchbenefitandcre

3、atmoreeconomicprofitsButinalongtimelittleeffortisdevotedtoidentifyandisolatetheminorpotentialehemopreventiveagentspresentintheshellofcamelliaoleiferaabelandassesstheirproperties，duetoitstraceamountandcomplexityTheobjecti

4、veofthisstudyWastoisolateandidentifythebioactivecompoundsoftheextractofcamelliashellandtocharacterizetheantioxidantpropertiesandasaFASinhibitorofthesecompoundsTheoptimumextractingconditionsoftotalflavonesfromtheshellofca

5、melliaoleiferaabewerestudiedThebestconditionswgrechosenthroughorthographicexpeIimentsTheresultsshowedthat30：lof60％ethanolWasusedtodousethedriedtheshellofcamelliaoleiferaabewithultrasonictreatmentlastfor45raintwiceTheaver

6、ageextractionrateWasasllighas97，34％Thecontentofflavouesintheextractionliquidwasashighas171％Toevaluatetheirantioxidantactivities，weusesthreesystemslikedpph，frapand’OHscavengingcapabilityResultsshowsthatinDPPHsystemthe4006

7、ethanolextracthaveagoodantioxidantactivity，itsICsois781tg／mL，lowerthanbambooextractwhichIC∞is85999／mLandobviouslylowerthantheotherextract；InFRAPsystem，theFRAPvalueofwaterextractis179mmol／Lwhichisveryclosetobambooextractb

8、utlowerthanVe；In‘OHscavengingsystem，whenalltheextractunderthe5賊／mLscavengingratefor40％ethanolextracLethanolextractandbambooextractis328％，381％，40％respectively，butlowerthanVcHPLCanalysisshowthatcamelliashellmayincludeQuerc

9、etinandRutinwhichhasacontentof425mg／kgand1478，5mg／kgrespectivelyExperimentsalsoshowthattheextraclofcamelliaseedshell(CSS)inhibitedFASactivitywithstrongreversiblefastbindinginhibition，／’’40％烈hanolWastheoptimalsolventforex

10、traction，halfinhibitionconcentration(1C如)ofCSStoFASoverallreactionWas202I_tg／mL，Wealsofoundtheextractofcamelliaseedresidue(CSR)inhibitedFASactivitywithirreversibleslow—bindinginhibitioninthepresenceofO31mg／mLofSCRtheinhi