檢測糞便中K-ras突變及胰液細(xì)胞學(xué)輔以檢測抑癌蛋白表達(dá)在胰腺癌篩選和診斷中的價(jià)值.pdf

1、中國協(xié)和醫(yī)科大學(xué)博士學(xué)位論文檢測糞便中Kras突變及胰液細(xì)胞學(xué)輔以檢測抑癌蛋白表達(dá)在胰腺癌篩選和診斷中的價(jià)值姓名：徐彤申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：消化內(nèi)科指導(dǎo)教師：陸星華錢家鳴2000.5.1DetectionofKrasmutationsinstoolsanddetectionofantioncogeneproductsasanadjunctincytologicexaminationofforscanninganddiagnosispu

2、repancreaticjuiceofpancreaticcarcinomaABSTRACTSBACKGROUND：1Pancreaticcarcinomaisconsideredasoneofthemostmalignanttumorinhuman，withthe5yearsurvivalratelessthan5％Inrecentyears，itsincidencewasincreasingsteadilyEarlydiagnosi

3、scanmakemoretumorresectableandimprovetheoutcomeofthisdiseaseKrasisthoughtspecificallyrelatedtothetllmorigenesisofpancreaticcarcinomaThepointmutationofcodon12inexon1ofKrasgonehavebeenidentifiedin90％ofpancreaticctrcinoma，b

4、utmuchlessinbenignpancreaticdiseaseSodetectionofKrasmutationmayassistthediagnosisofpancreaticcarcinomaOne0fourobjectsiStodetectKrasmutationsinstoolSanddisccussitsapplicationinscreeningforpancreaticcancers2!Theabnormaliti

5、esofgeneandexpressionofCDKinhibitorssuchadp53、P16Ink4、p21WallhaverecentlybeenimplicatedinpancreaticcarcinogencsisTherateofoverexpressionor10SSofexpressionoftheseantioncogeneproductsinpancreaticcarcinomawas37—79％Buttheexp

6、ressionofp27andtheinterrelationshipoftheseproteinsarestillunknown，CytologicexaminationofpurepancreaticjuicecanfindtumorCellsandhelptodistinguishbenignfrommalignantpancreaticdiseaseButthesensitivityoftheconventionalcytolo

7、gicexaminationwasunsatisfactoryTheseDondaimofthisstudyiStoinvestigatetheexpressionofantio硎cogeneproductsinpancreaticcarcinomaandbasedontheseresultstodevelopanewmethodofimmunOcytOchcmistryinconjunctionwithconventionalcyto