MAPK信號(hào)通路介導(dǎo)的氧化損傷對(duì)晶狀體上皮細(xì)胞水通道蛋白表達(dá)的影響.pdf

1、分類號(hào)：R776.1密級(jí)：不保密UDC：UDC：617學(xué)校代碼：11065碩士學(xué)位論文MAPK信號(hào)通路介導(dǎo)的氧化損傷對(duì)晶狀體上皮細(xì)胞水通道蛋白表達(dá)的影響MAPK信號(hào)通路介導(dǎo)的氧化損傷對(duì)晶狀體上皮細(xì)胞水通道蛋白表達(dá)的影響王曉川指導(dǎo)教師趙桂秋教授學(xué)科專業(yè)名稱眼科學(xué)論文答辯日期2015年6月05日EffectsofMAPKsignalingpathwayontheexpressionofaquapinsinducedbyoxidativeda

2、mageinhumanlensepithelialcellsAbstractObjective:Toobservetheexpressionofaquaprin1(AQP1)aquaprin5(AQP5)inhumanlenscapturehumanlensepithelialcells(HLECs)investigatetheeffectsofMAPK（mitogenactivatedproteinkinase，MAPK）signal

3、ingpathwayontheexpressionofAQP1AQP5inducedbyoxidativedamageinHLECs.Methods:Lenscapsulesfromagerelatedcataractpatientsafterphacoemulsificationdons?eyeswerecollected.TheexpressionofAQP1AQP5mRNAinthetwokindofcapsuletissuese

4、wasdetectedbyRealTimePolymeraseChainReaction(RTPCR).TheproteinexpressionlevelofAQP1AQP5wasdetectedbyimmunohistochemistry.HLECswereculturedinvitro.WedividedHLECsintocontrolgroupstimulatedbyH2O2groupinhibitpretreatmentgrou

5、promly.Westernblotwasappliedtodetecttheproteinexpressionlevel.RTPCRwasusedtodetecttheexpressionofAQP1AQP5mRNA.Results:(1)InthisstudyweconfirmedAQP5AQP1expressioninlenscapsuletissuseofcataractpatientsnmalinHLECsculturedin

6、vitro.(2)H2O2stimulationcanreduceAQP1AQP5mRNAproteinexpressionthedifferencewasstatisticallysignificant(P＜0.05).(3)TheexpressionofpERKpp38wasincreasedinducedbyH2O2(P＜0.05).ButtheexpressionofpJNKwasnosignificantlyincreased

7、(P0.05).(4)TheexpressionofAQP1inERKp38inhibitpretreatmentgroupwashighercompairedwithH2O2stimulationgroup(P＜0.05).HowevertheexpressionofAQP5hadnodifferenceinthistwogroup(P0.05).Conclusion:(1)AQP5AQP1areexpressedinlensepit

8、helialcells.ThelowexpressionofAQP5AQP1mayhaverelationshipwithcataract.(2)TheexpressionofAQP1AQP5isdecreasedinducedbyH2O2inHLECsthedecreaseofAQP1isinducedthroughERKp38signalingpathway.(3)MAPKsignalingpathwayhasnoeffectont