TGF-β誘導(dǎo)人肺腺癌細(xì)胞A549中血管生成相關(guān)基因ANGPTL4的表達(dá)上調(diào)部分依賴(lài)乏氧誘導(dǎo)因子HIF-1.pdf

1、華中科技大學(xué)碩士學(xué)位論文TGF-β誘導(dǎo)人肺腺癌細(xì)胞A549中血管生成相關(guān)基因ANGPTL4的表達(dá)上調(diào)部分依賴(lài)乏氧誘導(dǎo)因子HIF-1姓名：余靜申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：腫瘤學(xué)指導(dǎo)教師：袁響林2010-05華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 3Up-regulation of Angiopoietin Like 4 (ANGPTL4) Expression in Human Lung

2、Adenocarcinoma Cells Induced by TGF-β partly Depending on Hypoxia Inducible Factor HIF-1 Department of cancer center Tongji hospital Tongji medical college Huazhong university of science and technology Post-

3、 graduate: Jing Yu Tutor: prof. Xianglin Yuan Abstract 【 Objective 】 : To silence the expression of HIF- 1α in Human lung adenocarcinoma cell lines A549 by RNA interference, and initially discuss the func

4、tion of HIF- 1 in TGF- β- mediated up- regulation of ANGPTL4 expression. 【Method】: The HIF- 1α- shRNA and empty plasmids were amplified and purified. Then, they were transfected into the human lung

5、 adenocarcinoma cell lines A549. A549 cell lines transfected with HIF- 1α- shRNA plasmid(experimental group), A549 cell lines transfected with empty plasmid (negative control group) and the A549 cell lines

6、untransfected with any plasmids were treated with TGF- β1 with the same concentration. Then, we use RT- PCR and Western- blot to examine the mRNA and protein expression levels of HIF- 1α and AN

7、GPTL4 in these three groups of cells respectively. 【Results】 : With the treatment of TGF- β1 for 0, 12, 24 and 48h, the expression levels of mRNA and protein of HIF- 1α and ANGPTL4 were sign