過氧化物酶體增殖物激活受體激動劑羅格列酮對人子宮肌瘤細胞的影響.pdf

1、山西醫(yī)科大學碩士學位論文過氧化物酶體增殖物激活受體激動劑羅格列酮對人子宮肌瘤細胞的影響姓名：秦忠芳申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：李美蓉20100316—————～一一生塑墾型奎蘭堡主蘭堡壟蘭Abstractob】eCtiVe：ToeXploretheleiomyomacellsgrowthi砌bitioniIlducedbyaperoxisomeproliferatoractlVatedrec印torYactiVatorro

2、si91itazonea11ditsmoIecularmechamsmandt0explore也eexpressionofPPARY、BaX、Bcl2耐醐A，andmesuppressivee虢ctofrosilitazone0nmeproliferationalld印optosis0futerineleiomyomacellsi11vinDOurp鄴se、懈toexplorethepossibIemecha血smofutemeIeio

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5、ctionaIldidemifiedbvimmunoc”ochemistⅨ(2)Mnmethodshowedt脅：Thetre鋤enta髓r24、48、72h(P005)Thetreatmem塒t1rosiglitazolle(5x106、5x10～、5lO培mo王／L)resuhedinaIli出bitionofmecell伊owms疵r72、48house(P(O05)，andmereweren0si班ficalltm酶enceb懶

6、eachotheriIlmetreatmentof24h(PO05)(3)S“。qu孤t堿VeRTPCRresuhsShowed恤t：Rosiglitazone(51驢、510～、5lO。8mo兒]線ctme幽eleiom歸acells72ll，奶nincreaSe齜expreSsionofPPARy，Bax腳ucemeeXpressionofBcl2，anditsroleisadosedependentmamler，showillg印

7、paremactivationinllibitionC0nCIuSion：(1)R，osigli屯azonesi髓ificantlyinllibitedtheinvi臼ocul眥dhuInaIlut謝neleiomvomacellproliferatio瑪withtlleincreaSei11drugdosealldtheextensionoftlletil：ne，ute血1eleiomvomacellproliferationanda

8、ctiVationareinhibited，showadoseandtimedependellt(2)Ros酒itazonecallsuppresst11eceJlp∞ji向ationpanly燦u曲出eregujati。11sofP隧R吖、BaXaIldBcl2expressionsTIlecrosstall【betweent11esi印alpath、vaysofPPAR吖andBaX、Bcl2maybeinvolvedinthepr