宮頸癌細(xì)胞周期及射線誘導(dǎo)的周期阻滯與放射敏感性的關(guān)系.pdf

1、華中科技大學(xué)同濟醫(yī)學(xué)院碩士學(xué)位論文宮頸癌細(xì)胞周期及射線誘導(dǎo)的周期阻滯與放射敏感性的關(guān)系姓名：張孟賢申請學(xué)位級別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：于世英2001.5.1蘭!登苧查蘭旦塑墮堂墮堡主蘭蘭竺笙苧EffectsofCellcycleParametersandRadiationInducedCellcycleArrestsonRadiosensitivityinCervicalCarcinomaOncologyDept，TonalMedi

2、calCollegeAffiliatedTongjiHospital，HuazhongUniversityofScience＆TechnologyPost鏟aduateMengxianZhangTutorProfShiyingYuIAbstraet]Purpose：Thepurposeofthisstudywastocladfytheeffectsofcellcycleparameters(s—phasefraction，SPFandD

3、NAploidy)andradiationinducedcellcyclearrests(includingG1arrestandG2／Marrest)onradiosensitivityandevaluatetheirrolesinpredictictingtheresponsetadiotherapyinCervicalCarcinomaMethodsandMaterials：twenty—fourpatientswithcervi

4、calcancerweretreatedbyradiotherapy限T)TissuesampleswereobtainedbypunchbiopsyfromcervicaltumorsofallpatientsbeforeRTandafterthefifthdoseof2Gyexternalwholepelvisradiation(totaldose，DTl0Gy)Samplesweresubjectedtoflowcytometry

5、analysisforSPF，DNAploidyandradiationinducedcellcyclearrests，andnickendlabelingforapoptosis(AI)，andcomputingthedoseforthetumorstodiminishtohalfsize(D05)bymeasuringthesizesofthetumorsonceaweekandplottingsizeagainstdoseResu

6、lts：TheeffectsofDNAploidyonAIandDO5hadnostatisticalsignificanceApositivecorrelationwasobservedbetweenSPFobtainedbeforeRTandD05(r=0533，P=0007)ThetumorswithG1arresthadhigherAIandlowerDO5thantumorswithoutG1arrestduringRT，th