根形態(tài)在玉米耐低磷中的作用及側(cè)根發(fā)育的蛋白組學研究.pdf

1、中國農(nóng)業(yè)大學博士學位論文根形態(tài)在玉米耐低磷中的作用及側(cè)根發(fā)育的蛋白組學研究姓名：劉燕申請學位級別：博士專業(yè)：植物營養(yǎng)學指導教師：張福鎖米國華20060601AbstractTherehavebeenextensivestudiesaboutadaptiveresponsesofplantstolowphosphorus(P)stressNevertheless，forcropsaimingtogeeingahigheryield，the

2、mechanismforefficientacquisitionofsoilphosphorusremainslargelyunkownInthepresentstudytwomaizeinbredspefficient181andpinefficient197，wereusedtocomparetheirmorphologicalandphysiologicalresponsestoPstarvationattheseedlingst

3、ageItturnedoutthatrootdevelopment，especiallylateralrootsdevelopment，isakeytraitintolerancetoPstressinthecalcareoussoilTherefore，181whohasrootwithhigherbranchingwaschosentostudytheproteomicexpressionprofileofrootsgrownund

4、erdifferentphosphorusavailabilityConsideringthepivotalroleoflateralrootsinphosphorusacquisition，lateralrootinitiationmutantruralwasusedtoidentifyproteinsrelatedtolateralrootinitiationinmaizeFurthermore，togetabetterunders

5、tandingtolateralrootinitiation，wetriedtocreateareferencemapofproteinsexpressedinpericyclecellswherelateralrootinitiatesThemainresultsareasfollowed：1RootadaptiveresponsestoPstarvationaresignificantlydifferentbetweenmaizeg

6、enotypesThePefficiencyof181seedlinghadalargerrootsystemwhichallowedittoexplorealargersoilvolumeItalsohadstrongerrhizosphereacidiccapabilityandhigeracidphosphataseactivitybothinthcrootsandontherootsurfaceafterPdeficiency2

7、EnvironmentalphosphorusavailabilityalteredproteinexpressioninmaizerootAmongtiredifferentiallyexpressedproteins，6proteinswerefoundupregulatedbylowPstressTheseproteinswereinvolvedinmetabolism(putativeleucineaminopeptidase)

8、，energy(fructosebisphosphatealdolaseandputativemitochondrialATPsynthase)，signaltransduction(proteinkinaseCinhibitor)andcellulartransport(smallGTPbindingproteinRab2andADP—ribosylationfactor)Theproteinlsopentenyldiphosphat

9、edeltaisomerase2wasdownregulatedbyPstarvationThisproteinisknowntoinvolveincytokininsynthesis，aprocessbelievedinvolvedinPisignaltransductionandrootarchitectureregulationTheseresultssuggestedthatplantrooKscansensePichangei

10、ntheenvironmentandstartexpressionofproteinsinvolvingdifferentpathways3ForteenproteinsweredifferentiallyexpressedintheprimaryrootsofrumImutantandwildtype25daftergerminationSeveralproteinswhichmayhelpprimaryrootresistdamag

11、esduetolateralrootsemergingwerelackordecreasedsignificantlysuchasUDP—gIucosyItransferaseBX9，glutathione—Stransferase，HSP70homologandthiaminebiosyntheticenzymeWhilebetainealdehydeDHmaybeaneffectiveosmoprotectantinassistin

12、grumltotoleratecold，nutrientandwaterstress，anditwasexclusivelyexpressedinrumIprimaryrootsCinnamoylCoAreductaseinvolvedinligninbiosynthesisdisplayedareducedexpressioninruml，incontrast，aputativeI，4benzoquinonereductasewhic

13、hisknowntoplayapivotalroleinlignindegradationwaspreferentiall37accumulatedinrwnlInaddition，NAD—dependentisocitrateDH，succinateDI1andATPsynthasep—SUwerepreferentiallyexpressedintheprimaryrootofthemutantruml，whichmayimplyh