滋養(yǎng)細(xì)胞侵潤行為的基因調(diào)控失常與妊高征發(fā)病關(guān)系的初步研究.pdf

1、上海第二醫(yī)科大學(xué)博士學(xué)位論文滋養(yǎng)細(xì)胞侵潤行為的基因調(diào)控失常與妊高征發(fā)病關(guān)系的初步研究姓名：喬寵申請學(xué)位級別：博士專業(yè)：婦產(chǎn)科學(xué)指導(dǎo)教師：林其德2003.5.1上海第二隧科大學(xué)博士學(xué)位論文AbstractThepreliminarystudyoRtherelationshipbetweengeneticcontroldysfunctionofWesuccessfullyclonedtheencodingsequenceofmetastas

2、issuppressorgene—|≤SS1fromhumantermplacentatissueandconstructeditsprokaryoticexpressionplasmidpQE30／KiSS1byusingthegeneticengineeringtechniqueBothmRNAandproteinexpressionlevelsandtheirlocalizationsofMMP2，MMP9TIMP1，TIMP2a

3、ndKiSS，1inplacentaltissueof140als鵲ofnormalpregnantwomenf100casesofthefirsttrimester10easesofthesecondtrimesterand30找lsesofthethirdtrimester)ands7錢Isesofpatientswithvra00CflSesofmild，10casesofmoderateand17casesofseverePIH

4、)weredetectedbysemiquantitativeRTPCRanalysis，Hybridizationilsitu，WesternblotanalysisandImmunohistochemistryWefoundthattheoppositesup靜ioritytransformationoftheinvasionrelatedgene(MMP9)andtheinvasionsuppressorgenefrDaP1，TI

5、MP2andKiSS。1)intranscriptionallevelduringtheparticularperiodofthefirsttrimesterregulatedtheinvasionoftropheblastprecisely，whichmayplayanimportantroleinthefbrmationanddevelopmentofplacentaAscomparingwithnormalpregnancyThe

6、mRNAandproteinsexpressionsofinvasionrelatedgene娜MP9MMP2)weredemeaseda翻thoseofinvasionsuppressorgenefmss。1一TIMP1)increasedincasesofPIHThesegenesexpressionabnormalitywerecorrelatedwiththe～———————————————————————^m—————————