羅漢果多糖成分及其藥理活性的研究（Ⅰ）.pdf

1、廣西師范大學(xué)碩士學(xué)位論文羅漢果多糖成分及其藥理活性的研究（Ⅰ）姓名：陳海燕申請學(xué)位級別：碩士專業(yè)：有機化學(xué)指導(dǎo)教師：義祥輝20020101羅漢果多糖成丹及其藥理活性的研究(I)papefTheSGPSwereex仃actedwithheatinginawaterbath，oramicrowaveoven，oraultrasonicinstr哪entT王lroughonhogonalexperiments，meoptimunlcondit

2、ionsofwaterbathextractingwereasf0110ws：extmctingtemperature100℃，extracting3times，eachti訊e15hour，‰f，f口。97臼JVP門。，ff／water1：2(w，V)；theoptim啪conditionsofmicrowaveextrac“ngwefeasf01lows：m；cmwaveheating15mjn，ex打acting7“mes，所m即

3、插肼FvPHd所／water1：6(w／V)；theoptimumconditionsofultrasonicextractingwereaSfollowed：theultrasonictime60min，extrac“ng7times，所陽f，脅昌mrw刀口rff／water1：6(W，V)Undertheseoptimumconditions，t11eyieldofp01”accharideswas060％，124％and0741％

4、，respectiVe】yThecrudepoly5ach州desweredeproteinzatedbySeVagmemodThentheywere口urifiedonDEAEcellulose，f01lowedbyanelution“thwater，aqueoussodiumchloride(O1M，O5M)Fractionswereex鋤jnedwithphen01一sulfmicacidmethodThefbctionswere

5、di“dedintothreegroups，namedasPSJ，PS2，PS3ThePSlandPS2weremnherp1】ri矗edbyacolumnckomato掣amofDEAE—cellulose，SephadeXG一200togettheSGPSlandSGPS2respectiVelyTheirhomogenitywereproVedandtheirmolecularweightsweremeasuredbyHPLCon

6、D10L一150columnTheirMwwere470000，790000respectivelyAccordingtoTLC，theSGPS1wascomposedofD唱alactose，D—xylose，D—mannose，L—arabiose，L—rhamnose鯽dt、vounkoweduronicacjdTheSGpS2wascomposedofLrhamnoseandunkoweduron|cacidUV’IRandNM

7、RwereusedtoanalysestnIctureofSGPSlandSGPS2inthispaperTheph姍acologicalexperimentshowedthatcrudep01ysaccharidescouldpromoteir啪unesystemKEYWORDS：毋r口if婦g陽svP“Driif孤，加gf砂cJi歷℃y：P01ysaccharides；1solation＆Purincation；StructureA