熱休克蛋白27在脊髓缺血再灌注中表達(dá)的實(shí)驗(yàn)研究.pdf

1、Y1405307稻蔓哼籍髫倪碩士學(xué)位論文熱休克蛋白27在脊髓缺血再灌注中表達(dá)的實(shí)驗(yàn)研究TileGeneExpressionExperimentalStudyofTheHotShockProtein27IntheSpinalCordIschemiaReperfusionhuury學(xué)位類型專業(yè)躬稱研究生姓名導(dǎo)師蚓r名和職杯臨床醫(yī)學(xué)瓠【k學(xué)位中醫(yī)骨傷科學(xué)張炯集張建新任巨師TheGeneExpressionExperimentalStudyof

2、TheHotShockProtein27IntheSpinalCordIschemiaReperfusionInjuryMajor：0rthopaedicsandTraumatologyofTCMPostgraduate：ZhangjiongjiTutor：professor：ZhangjianxinAbstractAim：Studiesthehotshockprotein27throughtheRTPCRlawandtheimmuno

3、histochemistrymethodtothespinalcordischemia—reperfusioninjurytopourintothedifferenttimeintervalgeneandproteinexpressionsituation，furtherelaboratedthehotshockprotein27biologiescharacteristicsintheprotectionmechanismofthes

4、pinalcordischemia—reperfusioninjuryMethods：Theexperimentto2006。07／09completestotheFujiantraditionalChinesemedicinecollegelaboratorySDbigmouse48，arefemaleandmaledoesnotlimit，bodyweight180320g，average230g，Stochasticallydiv

5、idesintothenormalcomparisongroupandthespinalcordischemiareperfusioninjury0，1，2，4，8，12，24hgroup(n=6)，UsesZivinandSOonthemethodpreparationthemodelofthespinalcordischemia—reperfusioninjuryAfterlacksblood30mintopourintothece

6、rtaintimeagain(0，1，2，4，8，12，24h)Poursintofixedlywith4％paraformaldehyde，ExcisestheL34doublesidecentrum，fullyrevealedL34hardmembranepouch，shutsofftheperipheralnerveroot，takesouttheidenticalsectionspinalcord，UseingtheRTPCRr

7、eactingsystemandtheimmunohistochemistrymethodexaminestheHSP27geneandproteinexpressionofdifferentgroupsanddifferenttimecompartmentResults：48SDb遠(yuǎn)mouseenterstheresultanalysis，0)TheHSP27geneandproteinexpressionelevatesimmedi