小麥苗期熱脅迫基因表達譜分析及相關(guān)基因的克隆.pdf

1、中國農(nóng)業(yè)大學碩士學位論文小麥苗期熱脅迫基因表達譜分析及相關(guān)基因的克隆姓名：彭丹輝申請學位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：彭惠茹20050601AbstractWheatisoneofthemostimportantcerealcropsinChinaInrecentyears，theglobalaveragetemperaturehasincreasedduetotheenhanced“GreenhouseEffect’，heat

2、stresshascometobeoneofthesedousclimaticthreatsforwheatproductionandcausesscverceconomiclossesHoweverthemolecularbasisofcropsadaptationtohightemperatureisnotcompletelychmacterizedTheaimofthissmdyistoanalyzethegeneexpressi

3、onprofileofwheatinresponsetoelevatedtemperatureandisolatethegenesinducedbyheatstressTodefinewheatmolecularresponsetoelevatedgrowthtemperaturesgeneexpressionprofileswereexaminedsubjectedtoheatsUessbyusingAffymetrix22kBarl

4、eylGenechipcRNAde巾edflomheattreatedwheatseedling(38℃for1hoⅢ丑s)andunUeatedwheatseedUng(C碼wereusedtohybddizedtwochipsrespectivelyAsaresult8486probesetsproducedpresentcallsinth06etwochipscovered372％oftotal22840probesetsAmon

5、gthemapproximately105％(n=s92)probesetsweresignificantlyupordownregulated(璐tal12andpvalue‘o05)SendquandtstiveRTPCRandRealtimeRTPCRwut≈usedtoverifythehybridizationresultAmongthe12geneswehaveexamined，11genesexhibitedthesimi

6、larresultswiththechipsSowebelievethatthechipsresultiscredibleAnalysisshowedthedifferentiallyexpressedgenesInvolvedinStle镕response，signaltransduction，transport，photosynthesispxoteinmetabolism，l歸membolismandcarbohydratemet

7、abolismetcAsexpected，alargenumberofthegeneshomologoustoknownchaperonesandheatshockproteinsinotherorganismswerehighlyinducedInadditionserialpredictedgenesincludingthoseencodingkeycm呵m％inUbiqultinPmteasomepathwayhaddiffere

8、ntiallyexpressedThecompleteORFoftwoheatinducedgeneswegecloedbyinsilicocloningmethodBioinformaticanalysisshowedthatonesequenceisencodingacalciumdependedpmte／nkinase(CDPl0andanotherisencodingaSer／IhrproteinkinaseInaddition

9、onemyristoylationsitewasdetectedintheNterminalofthepredictedCDPKandtwonucleartargetingsignalsequenceswasfoundintheNqerminalofthepredictedSeffrhrproteinkinaseThisresultindicatethoseCDPKandSer／Thrproteinkinasewouldbeanchor