22303.線粒體生物發(fā)生和能量代謝相關(guān)基因在小鼠ips誘導(dǎo)過程的變化和機理的初步研究

1、中國科學(xué)技術(shù)大學(xué)碩士學(xué)位論文線粒體生物發(fā)生和能量代謝相關(guān)基因在小鼠iPS誘導(dǎo)過程的變化和機理的初步研究姓名：劉曉鵬申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：曾令文20110602AbstractIIABSTRACTAtpresent，thetechnologyofinducingadultothernonterminaldifferentiatedsomaticcellsintopluripotentstemcells(iP

2、SC)hasmatured.somepapershavereptedthattheiPSCcanbegeneratedfromlessthanthreefourfacts(SKOMSKO).Howeverbeinganewbntechniquetherearestillmanyproblemsaboutthemechanismofreprogrammingwestillhavenotclearlyunderstoodespecially

3、thecentralissueofthereprogrammingmechanismispolyunderstood.MitochondriaplayaveryimptantroleintheprocessofiPSCsinductionbesidesmphologyfunctionofmitochondriahaveundergoingsignificantchangesthesechangescouldmeettheneedofiP

4、SCs.HerewegeneratediPSCsfrommousefibroblastftheanalysisofmitochondriabiogenesisrelatedgenesexpressionitsimpactontheiPSCsreprogramming.OntheonehduringtheprocessofiPSCsgenerationmitochondriachangesconsistentwiththeiPSCsind

5、uction.OntheotherhchangesofmitochondriabiogenesismayenhancetheiPSCsreprogramming.Wescreenedaseriesofgenesaboutglycolysisoxidativephosphylationmitochondrialbiogenesistrytofindsomegenescanmakesomechangesduringthereprogramm

6、ing.AccdingtotheexperimentsresultswefoundthattheinhibitionofmitochondrialbiogenesisgenePGC1canenhancetheefficiencyofiPSCsgeneration.therearesomeproofsthattheincreaseoftheexpressionofPGC1couldlowertheiPSCsgeneration.Anywa