28819.噬菌體內(nèi)溶素基因分離、鑒定及功能研究

1、（主題:類別:全日制碩士研究生全日制碩士研究生題目:噬菌體內(nèi)溶素基因分離、鑒定及功能研究噬菌體內(nèi)溶素基因分離、鑒定及功能研究英文題目英文題目:Studyonseparationdeterminationandfunctionofphageendolysingene研究生研究生:王學(xué)琴王學(xué)琴學(xué)科名稱學(xué)科名稱:生物化工生物化工指導(dǎo)教師指導(dǎo)教師:劉麗華劉麗華副教授副教授二○一○年五月二○一○年五月碩士學(xué)位論文碩士學(xué)位論文分類號(hào):學(xué)校代碼:10

2、128分類號(hào):學(xué)校代碼:10128UDC:學(xué)號(hào):20071171學(xué)號(hào):20071171AbstractWithincreasingofbacteriaresistancetoantibioticsitisurgenttofindanantimicrobialagentthatiseffectiveuneasytocreateresistant.Theresultsshowthatthephagephageencodedlyticenzy

3、mesarethemostidealsubstituteofantibioticsftreatmentpreventionofbacterialinfection.Becausetheyhaveimptantvalueondrugresistanceofantibacterialscreeningproteinswithantibacterialactivityfromthephageisanewapproachmethod.Inthe

4、studyafiercebacteriophagenamedEnterobacteriaphageWL08wasisolatedfromthechickenmanuregene23encodingcapsidproteinofEnterobacteriaphageWL08was96%similartogene23ofEnterobacteriaphageRB69.Egenethatencodesendolysiswascloned.Se

5、quenceanalysisshowedthatthisgeneconsistedof474bpencodinga157aminoacidproteinwith98%similaritywiththeEgeneofEnterobacteriaphageRB69.ThenEgenewastransfmed:FirstofallEgeneRIgenewerebridgedby(GlyGlyGlyGlySer)3toobtainRIEgene

6、secondlyEgenewastransfmedthroughPCRamplificationtoobtainE187E34157E110157.TheninhibitioneffectofRIEEE187E34157E110157proteinonthegrowthofE.coliwasmanifestedbycloningRIEEE187E34157E110157geneintothepHERD30Tvectswhichconta

7、insaPBADpromoter.TheresultindicatedthatRIEEE187E34157E110157proteinshowedthebacteriostaticactivityagainstgrowthofE.coli.ToexpleanewapproachofexpressionofEproteininE.coli2566EgeneobtainedbyPCRwasclonedintothevectpTYB11toc