利用微衛(wèi)星標記定位太空誘變玉米細胞核雄性不育基因研究.pdf

1、四川農(nóng)業(yè)大學碩士學位論文利用微衛(wèi)星標記定位太空誘變玉米細胞核雄性不育基因研究姓名：黃文超申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：榮廷昭曹墨菊20030501AbstractItisallimportantmethodofobtainingheterosisbyusingmaizemalesterilityThemaizecytoplasmmalesterilematerialsareinfectedeaslybyCornsouth

2、ernleafblight，SOthesearchofmaizemalesterilitywastransferredfromcytoplasmmalesterilematerialstonuclearmalesterileitymaterialsWesentmaizeseedsforsatelliteaboardin1996andgotanuclearmalesterilemutantintheoffspringofthesingle

3、hybrid—chuanDanno9Inordertoanalysisthismaterialthesistercrosspopulation(RP3195(A)RP3195l(B))wasdetectedwithSimpleSequenceRepeatmoleculermarkerThemainresultsconcludedasfollows1Thereareseventyninenormalfertileplantsandsixt

4、yninemalesterileplantsinthepopulationthetraitofmalesterilitywascontrolledbyasinglerecessivegeneinthechromosome2TheanalysisofSimpleSequenceRepeatshowsthatnodifferencebetweennormalfertileDNAbulkandmalesterileDNAbulkwasdete

5、ctedby517pairSSRprimers，but3outofthe517SSRprimerstookonpolymorphsmswhenusesinglenormalfertileplantandsinglemalesterileplantDNAasPCRtemplation3Whenampplyiedthesepolymorphicmarkerswitheveryplantinthesegregatingpopulation，o

6、nlythemicrosatellitelocusbnlgI141whichmappedonchromosome2Linmaizetookonco—segregatingwiththefertilityexpressionofeachplantinthesistercrosspopulation，SOthisresultsuggestedthatthelocusofbnlgl141maybelinkedwiththemalesteril

7、egenems，accordingtotheMapmakersoftwareanalysis，thegeneticdistancebetweenbnlgll41andthemalesterilegenemswasestimatedtobe353cM4ThestrategyoftouchdownPCRwasusedinthisresearch，comparedwiththeusuallyPCR，ithasabetterspeciality