Bt基因?qū)氚撞祟愂卟说难芯?pdf

1、華中農(nóng)業(yè)大學碩士學位論文Bt基因?qū)氚撞祟愂卟说难芯啃彰和醴迳暾垖W位級別：碩士專業(yè)：蔬菜學指導教師：葉志彪2002.5.1華中農(nóng)業(yè)大學碩士學位論文GeneticTransformationofvegetableBrassicacampestriswithBtgeneSpecialty：VegetableScienceMasterDegreeStudent：WangFeng，Supervisor：ProfYeZhibiaoNationa

2、lKeyLaboratoryofCropGeneticImprovementCollegeofHortcultureandForestryHuazhongAgriculturalUniversityWuhan，PRChina(43007)AbstractBrassicacampestrisLssppekinensisBrassicacampestrisLsspchinensisandBrassicacampestrisLsspvarpa

3、achinensisBaileysynchinensisareimportantvegetablecropsinChinaInsectpestssuchasmothposeasevereproblemintheproductionofvegetableBrassicacampestrisworld—wideTheconventionalinsectcontrolmethodismainlydependentonintensiveande

4、xtensiveuseofchemicalpesticideswhichhavedrawbackssuchasdamagetotheecologicalsystem，residualpoisoningofhumansandanimalsMoreover，thepesthasdevelopedresistancetomanyofavailableinsecticidesTherefore，itisdesirabletodevelopins

5、ectresistantplantsPlantbreedersinthepastseveraldecadeshaveusedinterspecificsexualhybridizationtotransfergenes，butsexualincompatibilitybarriersandlackofinsectresistanceplantparentsseverelylimitthepossibilitiesforgenetrans

6、ferbetweenspeciesGenetictransformationtechniquesopenedanewavenueforcropimprovementandprovidednewapproachestobreedingobjectiveofinsect—resistantBacillusthuringiensiscrystalproteingeneshavebeenproveneffectiveincontrollingi

7、nsectlarvaeinmanycropplantsTransgenicvegetableBrassicacampestrisexpressingBacillusthuringiensisendotoxinsofferanalternativeinsectcontrolmethodIncontrasttothesesuccessfullytransformedBrassicaspeciesvegetableBrassicacampes

8、trisaregenerallyconsideredtoberecalcitranttoshootregeneration，andonlyafewreportsonthetransformationofthesematerialshaveappearedEfficientgenetransferandplantregenerationsystemsarenecessaryforthedevelopmentoftransgenicplan

9、tsInthisreport，regenerationsystemofBrassicacampestriswasestablishedWealsodescribedanefficientprocedureofgenetransfersystemviaAgrobacteriumtumefaciensinthedevelopmentofinsectresistantplantsThemainresultsareasfollows：1One—

10、stepregenerationsystemforcotyledonofBrassicacampestriswasestablishedwiththecombinationofBA，NAAandAgN03MaximumshootregenerationratesofBrassicacampestrisLssppekinensisBrassicacampestrisLsspchinensisvarpaachinensisBaileysyn