無角山羊間性綜合征缺失片段及豬?；蚪M中長(zhǎng)散布元件的序列分析.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文無角山羊間性綜合征缺失片段及豬?；蚪M中長(zhǎng)散布元件的序列分析姓名：李東鋒申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：李祥龍20090606SequenceAnalsysoftheDeletionRegioninPISGoatsLINEsinPigBovineGenomeAuth:DongfengLISpecializedfield:GeicsTut:Pro.XianglongLIAbstractA~11.7kbdel

2、etionreigntriggersintersexualitypollednessingoatstermedasPolledIntersexSyndrome(PIS).Throughthesequenceanalysisoffourrepeatedsequences(13120bp，39884565bp，49897088bp，78759135bp)ingoatPISdeletionregion(AF404302)wefoundthat

3、the78759135bpsegmentbelongedtoL1M3subfamilyofLINE1theothersweremembersofBDDFsubfamilyofRTE.DependingontheresultsoftblastnprogramsearchingusingthefulllengthproteinsequencesofRTdomaininPISregionasquerysequencesagainstthebo

4、vinegenomedatabase56consensussequenceswereconstructed.UsingtheconsensussequenceswededucedthefmationtimeofPISregionat22.530MyajustthedivergencetimeofBovidaefromArtiodactyla.CombinedwithotherevidencethePISregionmightonlyex

5、istinBovidae.WedetectedthecontentdistributionofL1RTEinbovinepiggenomefthefirsttime.TheaveragecontentlengthofL1is7.89%529bpinbovinegenome10.64%608bpinpiggenome.TheaveragecontentlengthofRTEinbovinegenomeis11.83%443bp.Pigge

6、nomedoesnotcontainRTE.ThecontentofL1RTEinbovinepiggenomesreducedwiththeincreasingoftheGCcontentofeverychromosome.DependingonthenucleotidedivergencewedividedtheL1inbovinepiggenomeinto6families.ThebovinegenomecontainsL1_BT

7、1L1_BT2L1_BT3L1_BT4L1_Art1L1_Art2thepiggenomecontainsL1_SS1L1_SS2L1_SS3L1_SS4L1_Art1L1_Art2.InwhichL1_Art1L1_Art2arethecommonsequencesinArtiodactylsanimalgenomestheothersareonlypresentintheirowngenome.Becausetheconsensus

8、sequencesofRTEareveryyoungwiththesimilaritymethan90%piggenomedoesnotcontainRTEsequence.CombinedwiththeRTEsofotherspecieswededucedthattheRTEinbovinegenomeiginatedfromanunknownspeciesbyhizontaltransferdidnotfromtheantiquem