不同免疫刺激劑對(duì)水產(chǎn)動(dòng)物免疫功能影響的研究.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文不同免疫刺激劑對(duì)水產(chǎn)動(dòng)物免疫功能影響的研究姓名：劉勇申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：陳昌福楊季芳20070601華中農(nóng)業(yè)大學(xué)2007屆碩士論文Abstract1Channalcatfish(尼t(yī)alurllspunctatusRafinsque)wereimmunizedwithcrudelipopolysaccharide(LPS)extractedfromCytophagacolumnaris，Ae

2、romonashydrophilaandEdwardsiellaictaluriTheimmunogenicityofcrudelipopolysaccharidetochannalcatfishwerecomparedbyexamingtheagglutinatingantibodytiters，crossag百utinatingantibodytitersofSerum，thephagocyticactivityandbacteri

3、cidalactivityofphagocytesinthebloodandheadkidneyTheexperimentalresultsshowedthattheag毋urinatingantibodytitersandcrossag醇utinatingantibodytitersofimmunizedchannelcatfishwerehi【gherthanthecontrolgroup，andthephagocyticactiv

4、ityandbactericidalactivityofphagocyteSinthebloodandheadkidneyofchannelcatfishwassignificantlyboostedandhadahighlevelimmuneresponseThecrudeIJSofthreepathogenicbacteriawerethoughttohaveacomnlonprotectiveantigenwhichcau∞acr

5、ossimmuneresponsetochannelcatfish2Carassiusauratuswasimmunizedby蛔ectingwithlipopolysaecharide(LPS)outermembraneprotein(OMPlextractedfrompsychrophilicbacteriaDifferenttimeaftertheiniection，influenceoftwoantigensonimmunolo

6、gicalfunctionofCarassiusauratuswasdetetrainedbytestingthelysozymeactivity，antibactericialactivity，phenoloxidaseactivityinserum，thePPandPIofwhitebloodcell，andthenumberofleucocytesehangeinbloodofCarassiusauratusTheresultsh

7、owedthattwoantigenshavehigherimmunogenicitycomparedwiththeexamingofthecontrol，thedifferencesbetweenthevariousindexwereverysignificanthigheL3BacterialflourwasusedasafoodadditivetotesttheimmunefIlnctionofMacrobrachiumnippo

8、nensisTheresultsshowedthatthePo，Ua，U1andphagocyticactivityofthetestgroupsaresignificantlyhigherthanthoseofthecontrollgroupsaftertheAeromonashydrophilaWasiniectedtotheMacrobrachiumnipponensis，whichcanstrengthentheirresist

9、anceagainstAeromonashydrophilaindicatingtheeffectivenessofbacterialflouronenhancingPo，Ua，UlandphagocytieactivityofMnipponense，leadingtoanincreaseintheimmunnologicalactivitiesFurtherstudiesareneededtoidentifythemechanismo

10、fbacterialflourinimmunefunction4T0determinetheeffectsofimmunitystimulantsoninnateimmuneresponseofCarassiusauratus，fishwerefeddietscontaining0(contr01)，02％，05％，08％immunitystimulantsfor1，4，7，14and21daysTheresultsshowedthat

11、thephagocyticactivitiesweregreatlyincreasedandthelysozymeactivitieswereobviouslyenhancedThissignificantdifiefenceofthelysozymeactivitiesdisappeared7dafterthefishstoppedfeedingmixeddiet(PO05)，butthissignificantdifferenceo