益生菌對致病e.coli黏附雞腸紋狀緣膜的抑制作用

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文益生菌對致病E.coli黏附雞腸紋狀緣膜的抑制作用姓名：許珂申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：魏萍20090620Abstract II Study on the Inhibition of Chicken Pathogenic E. coli Binding to Chicken Intestinal Brush Border Membrane by Probioties Abstract The s

2、tudy treated chicken Intestinal mucous membrane as the model and selected two probio- tics (L. acidophilus and L.casei) and an avian pathogenic E. coli as research objects, to investiga- te the competitive exclusion mec

3、hanism of probiotics by lectin-binding histochemistry ABC and solid phase binding and binding inhibition assay. Healthy new born broiler chicken were selected and divide into six groups in randomly to est- ablish experim

4、ental animal model.One group were normal bred, one administration avian pathoge- nic E. coli, other groups administration L. acidophilus、 L.casei、 mixture of L. acidophilus and L casei and mixture of L. acidophilus and L

5、.casei followed avian pathogenic E. coli in difference. All group animals Intestinal mucous membrane were studied with lectin- binding histochemistry ABC to validate probiotic whether or not have the capacity to change s

6、ome specific glycosylation state. In addition, intestinal brush border membranes and bacterial adhesions were extracted to do Solid phase binding and binding inhibition assay, and analyzed by Scatchard plot and Hill plot

7、. To inve- stigate the mechanism of probiotic inhibition pathogenic bacteria from molecular directions. The results showed that D-mannose conjugates in the brush border membranes was the main receptor of pathogenic E. co

8、li, small amounts N- acetylneuraminic acid conjugates also related L-fu- cose conjugates and N-acetylglucosamine wre possible the receptor of L. acidophilus. N-acetyl- neuraminic acid was the main receptor of L.casei, be

9、sides galactose conjugates also related the rec- eptor. Two probiotics and avian pathogenic E. coli all able to modify some specific glycosylation state E. coli could improve the expression of D-mannose lectin receptor a

10、nd N-acetylneuraminic acid l- ectin receptor in intestinal brush border membrane of all sections except ileum, but N-acetylneura- minic acid lectin receptor increase smaller; L. acidophilus increased the expression of L-

11、fucose lec- tin receptor and also N-acetylglucosamine lectin receptor; The expression of galactose lectin receptor and N-acetylneuraminic acid lectin receptor also improved by L.casei. Furthermore, the expression D-manno

12、se lectin receptor in chicken intestinal mucous membrane which fed probiotics all degraded a little, but difference quiet. The interaction of adhesions of two probiotics and fimbriae of E. coli with intestinal brush bord