纖維素分解菌的混合培養(yǎng)及互生特性研究.pdf

1、四川大學(xué)碩士學(xué)位論文纖維素分解菌的混合培養(yǎng)及互生特性研究姓名：龐良偉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：化學(xué)工藝指導(dǎo)教師：鐘本和20030503鱧殘大學(xué)蘸圭學(xué)位論文ThestudyofthemixedcultureandthemutualisticcharacteristicsofthecellulolyticstrainsChemicalTechnologySupervisor：ZhongbenheProfessorThispaperstudie

2、sthecharacteristicofthefourstrainsthatscreenedfromthedecomposedstrawandthesoiioftheox，andstudiestheconditionsofthefermentatjonandthesolidstateculturemediumthatthestrainscanwellgrowonThenwemixthestrainsbydifferentcombinat

3、ionsandfindoutthebestonethatcandecomposethecelluloseandstudythesymbiosisofthecombinationsThenWeculturethebestcombinationsonthesolidculture越ediummadeoftheconductoftheagriculture，andprovedtohavebestcapabilitytodecomposethe

4、strawFinallywefindoutthebettercombinationsandtheconditionoffermentationthatcanbeusedincommercialmanufactureFourstrainsnamedasMl，B3，S7，S12aleobtainedthatthesuitablepHvaluesshollldbe65，75，65and75Atthesametime，thefourstrain

5、sallgrowwellat30～40“Cwithenoughoxygen。AndtheS7hastheability協(xié)bearpreferabletemperatureM1B3andS7havethesameamountofenzymeintheeellandoutofthecellbutS12hasmoreintheeellthanoutofthecell。Theoptimizedrecipeofthesolidstatecultu

6、reofthestrainsiSobtainedasfollows：powderofthebean5％；bran，7S％ThestrainsgrowwellontheculturemediumforthreedaysTheoptimizedcombinationsareS7／S12andM1／S7，andtheoptimizedmixstraininoculationproportionistestedtobeS7／S12：1：l，M1