蠶蛹中高純度α-亞麻酸及氨基酸提取工藝的研究.pdf

1、蘭州理工大學(xué)碩士學(xué)位論文蠶蛹中高純度α亞麻酸及氨基酸提取工藝的研究姓名：呂秋楠申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：化學(xué)工藝指導(dǎo)教師：史高峰20090501蠶蛹中高純度a亞麻酸及氨基酸提取工藝研究量量量曼量置曼魯皇曼曼量鼉m—mml。。mi皇皇量曼皇量曼曼量皇量置量皇量量魯皇置魯—■置皇鼉量量量—一ABSTRACTSilkwormhasaveryconsiderablevalue，itisrichinprotein，fattyacids，chitin

2、andotheringredientswhichiSallextremelyrichandvaluablenaturalresourcesandhasawiderangeofuseinthefood，pharmaceuticalindustries，chemicalindustriesandotherindustriesThispaperdiscussedtheultrasonicextractingsilkwormchrysaliso

3、ilthroughthesinglefactortestsandorthogonaltest，fourparametersofextractiontime，extractiontemperature，ratioofmaterialtoliquid，ultrasonicpoweraffectingtheyieldofsilkwormpupaoilTheexperimentalresultsshowthatextractiontimeaff

4、ectedtheyieldofsilkwormpupaoilsignificantly，optimalconditionsofultrasonicextractingsilkwormchrysalisoilareasfollows：ultrasonicpowerisIOOW，temperatureis55℃，liquidratioof1：7，extractiontimeis6h，intheabovementionedconditions

5、，thepupaoilextractionrateof2967％Intheprocessobtainedthesilkwormchrysalisoilthatwasgoodcolor，lowodorontheconditionsTheuseofureapurificationofa1inolenicacidwerestudiedonthetrendsofthecontentandyieldof伍1inolenieacid，whichwe

6、reaffectedbytheratioofrawmaterialsadductiontime，adductiontemperatureWhenthecontentof伍一linolenicacidwasincreasing，theyieldofalinolenicacidwasdecreasingAsfollows：theradionoffattyacidtoureatomethanol(w／W／v)of1：3：10，adductio

7、ntemperatureof0℃。adductiontimeof12h，fattyacidwasadductedtwiceundertheconditions，thecontentofa1inolenicacidincresedfrom3745％to8387％，yieldofa—linolenieacidwas1826％Defattedsilkwormwashydrolyzedbyhydrochloricacid，theprocessi

8、ssimple，shortproductioncycle，lowcost，canbernorefullyhydrolyzedprotein，highyieldHydrolysisofthebesttechnologywassolid—liquidratioof1：6，hydrochloricacidconcentrationof6mol／L，thehydrolysistemperatureof103℃，thehydrolysistime

9、was18h0236molcompoundaminoacidswasskimmedfrom1OOgdefattedsilkworm，vacuumdryingthesolidwas31629，theyieldofcomplexaminoacidis3I62％Hydrolyzatewasdetreated2hbyadding2％ofactivatedcarboninawaterbathat80“CintheconditionsofpH=3R

10、esultofhydrolyzateisgoodHydrolyzatewaspurifiedby732strongacidcationexchangeresinAftervacuumdryingthefinaleluatewastastelesslightyellowcompoundaminoacidpowderThesolidphaseofureaclathratewassolvedbythewayofacidleachingunde