簡介：華中農(nóng)業(yè)大學碩士學位論文核盤菌弱毒相關病毒基因組分子生物學特性的初步研究姓名衛(wèi)冬妹申請學位級別碩士專業(yè)植物病理學指導教師姜道宏20030501華中農(nóng)業(yè)大學2003年碩士論文核盤苗弱毒相關病毒基因組分子生物學特性的初步研究PRELIMINARYSTUDIESONTHE64KBDSRNAGENOMEOFTHEMYCOVIRUSINFECTINGTHEHYPOVIRULENTISOLATEEP1PNOFSCLEROTINIASCLEROTIORUMABSTRAETINTHISDISSERTATIONTHE64KBDSR_NAGENOMEOFTHEMYCOVIMSINFECTINGTHEHYPOVIRULENTISOLATEEP1PNOFSCLEMTINIASCLEROTTORURASSHVWASSTUDIEDUSINGTECHNIQUESOFEDNASYNTHESIS，CLONING，ANDSEQUENCINGTHERELATIONSHIPBETWEENHYPOVIRULENCEOFSCLEROTINIASCLEROTIORUMEP1PNANDTHEDSR2QAMYCOVIRUSWASFURTHERCONFIRMEDATTILEMOLECULARLEVELRESETSACHIEYEDSOFARINCLUDELPOSITIVEEDNACLONESOFTHE64KBDS_RNAOFEP1PNWEREOBTAINEDAFTERSEQUENCINGTHEINSERTIALCDNAFRAGMENTS，INTHEPLASMIDPARTIALEDNASEQUENCEOFTHE64KBRNAGENOMEOF4629NUCTEOTIDESWASALIGNEDWITHDNAMANPROGRAMTHESEQUENCE4629BPWASSUBMITTEDTOTHEGENBANKWITHTHEACCESSIONNUMBERAYL472602AINCOMPLETEOPENREADINGFLAME0咖ENCODINGAFUSIONPROTEINOF1440AMINOACIDRESIDUESOFHELICASEANDRNADEPENDENTRNAPOLYMERASERDRPOFRNAVIRUSWASREVEALEDTHEGENECOATPROTEINAMINOACIDSSEQUENCEWASNOTINCLUDEDINTHISSEQUENCEBASEDONTHECHARACTEDSTIESOFPUTATIVEFUSIONPROTEINTHE64KBDSLLNAELEMENTCOULDBEREGARDEDASMYCOVIRUS，ANDNAMEDASSCLEROTINIASCLEROTIORUMHYPOVIRULENCEASSOCIATEDVIRUSSSHV3BOTHTHENUCLEOTIDESEQUENCEANDPUTATIVEAMINOACIDSEQUENCEWERESUBJECTEDTODOHOMOLOGYSEARCHWITHBLASTPROGRAMONHTTP／／WWWDDBJNIGACINTHERESULTREVEALEDTHATTHEOBTAINEDNUCLEOTIDESEQUENCEHADTHECHARACTERISTICOFPLANTPOTEXVIRUSSUBSEQUENTLYTHEPUTATIVEPROTEINCODEDBYTHE64KBDSRNAWASHIGHLYSIMILARTOTHOSEPROTEINSCODEDBYPOTEXVIRUSSUCHASBAMVBAMBOOMOSAICVIRUS，PVXPOTATOVIIUS蜀，CIYMVCLOVERYELLOWMOSAICVIRUSANDTUVXTULIPVIRUS均ETA1THERESULTSUGGESTEDSSHVMIGHTDERIVEFROMPLANTVIRUSORSHOWEDTHESAMEANCESTORWITHPLANTVIRUS4BASEDONTHEEDNASEQUENCEOFSSIWSPECIFICPRIMERSWASDESIGNEDTODETECTDSR_NAINEPIPNANDITSDERIVATIVESUSINGRTPCRTHERECOVEREDCULTURESINCLUDTHEEASCOSPOREPROGENIESEP一1PNAAEP1PNABANDEPIPNAE，THEEPROTOPLASTREGENERATIONCULTURES∞PIPNPL4，EP1PNP94ANDEP1PNL31ANDONESINGLESCLEROTIUMISOLATION2

簡介：浙江大學碩士學位論文葫蘆科植物病毒研究Ⅰ主要病毒鑒定與基因組分析Ⅱ病毒生態(tài)學和致病性研究姓名陳潔云申請學位級別碩士專業(yè)微生物學指導教師陳集雙20030501略語表AMP氮芐青霉索鈉鹽BP堿基對BSA牛血清蛋白BU束狀體CI柱狀內(nèi)含體CP外殼蛋白DDH20雙蒸水DEPCH20DEPC處理水DSRNA雙鏈RNAEDLA已二胺四乙酸ELISA酶聯(lián)免疫吸附測定ICTV國際病毒分類委員會IPL’G異丙基硫代．B一阱半乳糖苷KB千堿基對KDA千道爾頓NT核苷酸ORF開放閱讀框PCR聚合酶鏈式反應PVP聚乙烯吡咯烷酮PW風輪狀體卟PCR逆轉(zhuǎn)錄聚臺酶鏈式反應SDS十二烷基磺酸鈉SR卷筒體UTR非編碼區(qū)XGAI薯F寢’3。吲噼臥畔乳V¨IAMPJCILLINSODIUMSALTB∞EPAIRBOVJNESE門MAIBUMJNBUNDIECYLINDERICALINCLUSIONCOATPROTEINDOUBLEDJSTILIEDWATERDISTILIEDW蹴RTREATEDWITLLDEPCDOUBLE．S訂卸DEDRNAE岫LENEDJ姍JNETE打AACETICACIDER嗎恤ELJNKEDIMMUNOSORBENT邪SAYINCEMATIONA【∞MM；CTEEONTAXONOMYOFVJLLISESISOPROPYLMIOBDGAJACTOSIDEKIIOB鶴EPAIRKJIODALT∞NUCIEIOTIDESOPENREADING療1MEPOLYMER勰ECHAINREACTIONPOIYVINYLP”ROIJDONEPINWHEEIREVER北咖SCRIPTJONPOIYMERA轉(zhuǎn)CHAJDREACTI冊SODIUMDODCCYLSULFATESCM¨UNN鋤SIA鈀D障GION5．BMM一．CHOIORO_3INDOLYLBD驢L孔TOSIDC

簡介：山東農(nóng)業(yè)大學碩士學位論文中國傳染性法氏囊病病毒野毒株致病性的流行病學研究姓名孫淑紅申請學位級別碩士專業(yè)預防獸醫(yī)學指導教師崔治中200361山東農(nóng)業(yè)人學碩JJ學位論文2003IBDVVVIBDVELDJ。RTPCRSPFDEPCDDH0PBSSDS英文縮略表傳染性法氏囊病病毒超強毒傳染性法氏囊病病毒雞胚半數(shù)致死量反轉(zhuǎn)錄聚合酶鏈反應套式PCR無特定病原微生物焦碳酸乙二脂超純水磷酸鹽緩沖液十二烷基硫酸鈉

簡介：上海交通大學碩士學位論文雞傳染性支氣管炎病毒保守基因片段的克隆及分子生物學檢測技術的研究姓名張斌申請學位級別碩士專業(yè)生物化學與分子生物學指導教師華修國朱建國20040101上海交通大學碩士研究生畢業(yè)論文II14天M41組可從腎臟氣管肺臟和盲腸扁桃體中檢測到IBV存在肝臟不能檢出T株組可從腎臟肝臟肺臟和盲腸扁桃體中檢測到IBV氣管未檢出H120組只能從腎臟檢出其它臟器未檢出H52組從腎臟氣管和肺臟中可檢出其它臟器未檢出上海株組只能從盲腸扁桃體和腎臟中檢出IBV攻毒后21天可從M41和T組中腎臟檢出IBV攻毒后28天后從各組各臟器中均未能檢出IBV存在對照組均未檢出IBV這為研究IBV感染動物模型積累了一定的資料本研究還對一株雞傳染性支氣管炎病毒上海分離株SH的核蛋白基因進行RTPCR和序列測定分析并對其編碼蛋白進行了分析結果如下對上海分離毒株進行RTPCR特異性擴增獲得長約12KB的特異性條帶對上海分離株進行序列測定結果表明該片斷含有IBV核蛋白基因全序列其結構基因全長1227BP編碼409個氨基酸將上海分離株核蛋白基因序列與已經(jīng)在GENBANK中注冊的IBV其它毒株進行同源性分析結果表明SH株與M41H120GX298SAIBWJZJ971VICS株的核蛋白基因同源性分別為87588291287385和872,將SH株的氨基酸序列與參考毒株的氨基酸序列進行同源性比較結果如下M41株897H120株902GX298株912SAIBWJ株897ZJ971株89VICS株909本研究豐富了IBV分子流行病學資料為進一步研究IBV的分子水平檢測方法研制IBDNA疫苗提供了基礎材料關鍵詞雞傳染性支氣管炎病毒核蛋白基因NESTEDPCR

簡介：中國農(nóng)業(yè)大學碩士學位論文屠宰豬肝臟人病毒性肝炎的病原學初探和病理學研究姓名許江城申請學位級別碩士專業(yè)基礎獸醫(yī)學指導教師佘銳萍20040601ABSTRACTWEDESIGNEDTHESEEXPERIMENTSINORDERTOKNOWTHEMEETHYGIENESITUATIONANDDETECTVHLHEPATITISINTHESLAUGHTEREDSWINETHESERESULTSWILLBEHELPFULTOTHEADMINISTRATIONOFS、ⅣINESLAUGHTEREPIDEMIOLOGYANDVACCINESTUDYOFTHEVIRALHEPATITISWEHOPETHATTHERESEACHERSHOULDADVERTTOTHISPROBLEMWEDESINGED7EXPERIMENTSTOPROVIDEENOUGHEVIDENCE1SEROLOGYDETECTIONINSLAUGHTEREDSWINE2HISFILOGYANDPATHOLOGYSTUDYINSLAUGHTEREDSWINE3HBCAGANDHBSAGIRNMUNOHISTOCHEMICALDETECTIONINSLAUGHTEREDSWINE4APOPTOSISDETECTIONINSLAUGHTEREDSWINE5ELECTRONMICROSCOPEDECTIONINSLAUGHTEREDSWINE6HBVDNAPCRDETECTIONINSLAUGHTEREDSWINE7HCVIMMTMOHISTOCHEMICALDETECTIONINSLATLGHTEREDSWINE。RESULTTHEREARETOTAL136LIVERSAMPLESOFSLAUGHTEREDSWINE，ITWASFOUNDTHAT48CASESOFALIDETECTEDSAMPLES35％WEREPOSITIVEFORBOTHTHEHBSAGANDHBCAG25CASESF18％1APPEAREDPOSITIVEHBCAG，BUTNEGATIVEHBSAGINTHESE船VANTIGENHBSAG，HBCA曲POSITIVESAMPLES，WEUSEANTIBCL2／13AXANDTUNELMETHODTODETECTAPOPTOSISINTHESELIVERSAMPLESRHEREARE8SAMPLESANTIBAXPOSITIVEWITHBEL2NEGATIVE3SAMPLESANTIBD2POSITIVEWITHBAXNEGATIVENETUNELRESULTISTLLESALNEASANTIBAXRESULT8SAMPLESCORRESPONDINGPOSITIVE，PCRRESULTSHOWSDOPOSITIVESAMPLESINTHESELIVERSSOMEVIRUSPARTICLESALEALSOFOTRADEDUNDEREMTHEREARE5SAMPLESPOSITIVE8％OFTOTALTHESERESULTSINDICATETHATTHEREISHEPATITISVIRUSESPECIALLYHBVINLTHESESLAUGHTEREDSWINE’SLIVERS，BUTNOTTHESAMEASTHEHUMANHBVWESUGGESTTHATTHEPUBLICHEALTHDEPARTMENTANDHEPATITISRESEARCHERMUSTPAYATTENTIONTOTHISPROBLEMKEYWORDSSLAUGHTEREDSWINE，IMMUNOHISTOCHEMIEALMETHOD，APOPTOSIS，VIRALHEPATITIS，PATHOLOGY

簡介：東北農(nóng)業(yè)大學碩士學位論文黑龍江省部分地區(qū)PED流行特征及病毒生物學特性研究姓名郎景華申請學位級別碩士專業(yè)預防獸醫(yī)學指導教師李一經(jīng)仇波20031201STUDIESONTHEEPIDEMICCHARACTERISTICSOFPORCINEEPIDEMICDIARRHEAINSOMEAREASOFHEILONGJIANGANDTHEBIOLOGICALPROPERTIESOFITSPATHOGENABSTRACTPORCINEEPIDEMICDIARRHEAPEDISAHIGHLYCOOTAGINUSENTERICDISEASEOFSWINECHARACTERIZEDBYVOMITINGDEHYDRATIONANDAHIGHMORTALITYINPIGLETS11LEDISEASEHASBEENFOUNDINMANYCOUNTRIESINCLUDINGCHINA，ANDCAUSESECONOLILICLOSSESINPIGFARMINGINTHEPRESENTSH|DY∞ACCURATEDIAGNOSISHASBEENMADEFORFIVEPIGFARMSTHATOUTBROKEVIRUSASSOCIATEDDIANHNINPIGSONTHEBASEOFCLINICALDIAGNOSISPCRDETECTIONANDVIRUSISOFATIONANDTWOOFTHEMWEREIDENTIFIEDASPEDVINFECTEDFARMS11”TESUITSFROMTHECLINICALDAMSHOWEDTHATTHEMORBIDITYRATESARE7269％AND8995％INSUDDEDPIGLETS6807％AND75％INGROWINGANDFATTENINGPI貉WHILETHEMORTALITYRATESARE6288％AND8131％INSUCKLEDPIGLETS831％AND1037％INOTHERAGEGROUPSRESPECTIVELYONTHETWOFARMSALTHOUGHSWINEOFALLAGESARCSUSCEPTIBLETOTHISVIRALM危C的NTHEMOFT8LITYINPIGLETSUNDER4WOEKSISCA3MFTLONLYOBSERVEDN把OUTBREAKSUSUALLYOCCURDURINGTHEWMTERASWELLASTHEEARLYSPRINGANDTHEMAJORCLINICALSIGNSCANBEOBSERVEDASSEVEREWATERYORYELLOWISHDIARRHEAISOLATIONANDIDENTIFICATIONOFVIRUSWEREMADEOFTCONTINUOUSVEROCELLSLINEFECALSPECIMENWHICHAREPOSITIVEFORAMPL心YLQGMGANEOFPEDVWITHUESTEDPCILWMINOCULATEDONTHECELLSANDTHEBLINDPASSAGESWEREⅦ弧KNAKENUNTILTHECPESCOULDBE毹蜘ON18PASSAGES11”CELLSB∞OMCROUNDEDLYSEDANDOFFTHEWALLOFTLIEVESSELSFORTESTIFYINGTHEPRESENCEOFPEDISOLATEONVETOCELLSRTPCRTECHNIQUEWASUSEDTOAMPLIFYMGENEATTHE5，10，15AND20PASSAGES，ANDASPECIFICFIAGMENTOFPEDVMGENEWASAMPLIFIEDTHERESULTSHOWEDTHATTHEISOTATEHASADAPTEDTOVETOCELTSTWOPAIRSOFPLIMERSWEREDESIGNEDACCORDINGTOTHEPUBLISHEDMGENESEQUENCESOFPEDVCV777ANDBRL／87SUAINSWITHOLI9041ANDELIMER50S011IFEAPREDICTED6SLBPFIAGMENTOFTHEMGENEOFPEDVWASAMPLIFIEDFROMTHERNACXLR嘶EDFROMTHEFECALSAMPLESBYRT“％睡PCI乙ANDTHENCLONEDINMTHEPMDL8TVECTOR111ECOMPLETESEQUENCESOFTHEMGANEOFTHEPEDEPIDEMICSTRAINIS681NUCLENTIDESENCODES226AMINO∞IDSN“SEQUENCESANALYSISRESUITSINDICATEDTHATTHEMMLEOTIDESEQUENCEOFPED∞IDEMICUALFTHAS9809％HOMOLOS_“INCOMPARISONWITHTHATOFCV777。EXC≈PTFOR13NUCLCOTIDEDIFFERENCESINTHESITESOF37。47，138，179，192，213，269，285，348，529，579。618AND629ANDSHARE9794％HOMOLOGYCOMPAREDTOBRL／87EXCEPTFOR14NUCLENTIDEDIFFMMLCESINTHEVL

簡介：揚州大學博士學位論文鵝源新城疫病毒部分生物學特性鑒定及其囊膜糖蛋白基因序列分析姓名萬洪全申請學位級別博士專業(yè)預防獸醫(yī)學指導教師劉秀梵20020501揚州大學博士學位論文胞、網(wǎng)狀細胞、巨噬細胞的胞漿內(nèi)。在感染鴨的氣管、肺、食道、腺胃、法氏囊、腎臟、哈氏腺等組織中亦能檢測到病毒抗原，持續(xù)時間多為11D～15D，氣管、腎臟則可達19D以上，個別感染鴨的腸道里微弱的陽性反應；在肝臟、胰腺、脾臟、腦和心肌組織中未檢測到病毒抗原。結合免疫組化染色強度、病毒抗原存在時間及相應組織病變的嚴重程度進行比較發(fā)現(xiàn)，鵝源NDV對鵝、鴨的致病性和組織親嗜性存在較大差異。在體外試驗中，本試驗研究了鵝源NDV在雞胚成纖維細胞CEF培養(yǎng)物上的致病變特性和增殖動態(tài)，并將其與雞源、鴿源NDV的相應特性進行了比較。結果，鵝源NDV、雞源NDV及鴿源NDV感染CEF次代培養(yǎng)物后，在24H即開始有病變產(chǎn)生，最初各種毒株導致的病變相似，表現(xiàn)為細胞變圓、皺縮、折光性增強，但鵝源毒株感染的細胞單層病變進展緩慢，表現(xiàn)為細胞逐漸地變圓、浮起、崩解，并伴有大量合胞體形成在84～144H單層破壞、消失。麗雞源、鴿源毒株接種的細胞單層病變進展迅速，在36一48H即見單層有因細胞壞死、脫落而導致的空白區(qū)產(chǎn)生，60～84H單層即徹底破壞。對病毒接種后不同時間培養(yǎng)上清HA效價的測定結果表明，鵝源NDV接種后84～120H培養(yǎng)上清HA效價達到高峰，為5～8L092，而雞源、鴿源NDV接種后60～84H上清HA效價即達到高峰，但其最高效價只有4LO啦。本研究結果表明鵝源NDV可以在感染鵝的多種組織細胞中復制，導致以變性、壞死為主的廣泛的組織損傷；鴨對鵝源NDV具有較強的抵抗力，但病毒可以在感染鴨的一些組織中復制鵝源NDV在體外具有比雞源和鴿源毒株更強的導致細胞融合的能力。2鵝源新城疫病毒融合蛋白基因部分片段的克隆及序列分析用RTPCR技術擴增NDVF基因的部分片段，經(jīng)克隆、測序獲得了11株鵝源NDVF基因的部分5’端1700個核苷酸M序列，分析測定的序列及推導的相應氨基酸序列，并對鵝源NDV的基因型分類地位進行探討。結果表明，除J蚍，98／GO株以外，11株病毒中有LO株病毒F基因的核苷酸同源性大于97％，JS／2／98／GO株與這10株病毒F基因核昔酸的同源性為907～912％。11株病毒與標準強毒株F49E8F基因的同源性為853％～868％。

簡介：學校名稱垡主壅些盔堂學校代碼Q5Q皇丫662045分類號密級豬流感病毒的分離鑒定、HA基因的克隆序列分析和血清學研究SWINEINFLUENZAVIRUSISOLATIONANDIDENTIFICATIONCLONINGANDANALYSINGOFTHEHAGENEANDSEROLOGICALSTUDIES研究生導師指導小組伍銳金梅林教授陳煥春院士金梅林教授陳煥春院士郭愛珍教授何啟蓋副教授吳斌副教授方六榮副教授專業(yè)預防獸醫(yī)學研究方向病毒性傳染病學位級別碩士中國武漢二OO四年五月ABSTRACTSWINEINFLUENZAISAHIGHLYINFECTIOUSRESPIRATORYDISEASECAUSSEDBYSWINEINFLUENZAVIRUSAFFECTINGSWINEOFDIFFERENTSTAGES，SEXESANDBREEDSSWINEINFLUENZAWASFIRSTREPORTEDINUSAIN1918SHOPEISOLATEDANDIDENTIFIEDH1N1SUBTYPESWINEINFLUENZAFROMSWINEIN1930THEDISEASERESULTSECONOMICLOSEANDTHREATSANIMALHUSBANDRYANDHUMANHEALTHATPRESENT，ITWIDELYTAKESPLACEINMANYNATIONSANDREGIONSINTHEWORLDTHERESEARCHOFSWINEINFLUENZAHASBECOMEAHOTTOPICNOWADAYSSWINEINFLUENZABROKENINOURCOURTRYIN2002OURLABORATORYCARRIEDOUTISALATIONANDIDENTIFICATIONFROMCOLLECTEDCLINICALSAMPLESTHREESIVH3SUBTYPESTRAINSWEREIDENTIFIEDUSINGSTANDARDDIAGNOSTICMETHODSBESIDESBIOLOGICALCHARACTERIZATIONSWERESTUDIEDTOTHESEVIRUSTHESEISOLATESCANAGGLUTINATEREDBLOODCELLSCOMINGFROMDIFFERENTANIMALS，BUTTHEIRHEMAGGLUTININWERENOTSTABLETHESEISOLATESWERESENSITIVETOPHYSICALANDCHEMICALFACTORS，BUTWERESTRONGLYRESISTANTTOD刪ANDCOLDTHESEISOLATESAREHIGHLYINFECTIOUSTOCHICKENEMBRYOTHESEMICEANDPIGSEXPERIMENTLYINFECTEDWITHSWINEINFLUENZADISPLAYEDCLASSICRESPIRATORYSYNDROMESANDVISIBLEPATHOLOGYTHESWINEINFLUENZAVIRUSTHEFULLHALGENEWASAMPLIFIEDBYRTPCRWITHPRIMERSDESIGNEDSPEFICTOCONSERVEDHAREGIONTHERESULTSINDICATEDTHATTHREESWINEINFLUENZAHADHIGHLYHOMOGALARSEQUENCESWITHH5SUBTYPEAVIANINFLUENZAVIRUSMEANTIMETHERESULTSHOWEDTHATFUJIANISOLATEBELONGSTOTHESAMEBRANCHASAIVINDIRECTENZYMELINKEDIMMUNOSORBENTASSAY伍LISAOFSIVWASESTABLISHEDFORDIAGNOSESIVTHEBESTWORKCONDITIONWASDONE，MEANTIMESPEFLCITYANDREPRODUCIBILITYTESTWERECARRIEDOUTTHERESULTINDICATEDSIVELISAWEESTABLISHEDHASGOODSPEFLCITYANDREPRODUCIBILITYANDCANBEAPPLIEDTODIAGNOSESWINEINFLUENZATHERESULTSINDICATEDTHATOURNATIONHADEXISTEDANDPREVAILEDSWINEINFLUENZA，THATSWINEINFLUENZAHADBECOMESERIOUSDISEASETHROUGHOURASSAYMETHODWHICHHADBEENESTABLISHEDWITHTHEINCREASINGDANGEROFMULTIINFECTION，THESEROLOGICALSTUDYWASBEDONETHERESULTSSHOWEDSWINEINFLUENZAANDPCVORAPPHAVEHIGHLYINFECTIOUSPERCENT，THOUGHSWINEINFLUENZAANDPRRSHADLOWLYINFECTIOUSPERCENTKEYWORDSSWINEINFLUENZAVIRUS；ISOLATEANDIDENTIFY；BIOLOGICALCHARACTERIZATION；HAGENESWINEINFLUENZASERAEPIDEMICSURVEY

簡介：中國農(nóng)業(yè)大學博士學位論文甜菜黑色焦枯病毒和煙草壞死病毒的比較病毒學研究姓名席德慧申請學位級別博士專業(yè)生物化學與分子生物學指導教師于嘉林李大偉20040601中國教監(jiān)大學博士學位論文ABSTRACTABSTRACTLHAVESTUDIEDTHEBEETBLACKSCORCHVIRUS，XINJIANGBBSVXANDNINGXIABBSVNISOLATESANDHAVEFOUNDPATHOGENICITYDIFFERENCESBEDNCONTHESETWOSTRAINSTHECOMPLETECDNACLONEOFBBSVXWASOBTAINEDBYRTPCROFTHEXINJTANGRNACOMPARISONSBETWEENBBSVXANDBBSVNREVEALEDANUCLEOTIDESEQUENCEIDANTITYOF98，9％，ANDTHEGENOMICSTRUTTUREWASALMOSTUNIFO巾1HOWEVER，THREEADDITIONAINUCLENTIDESWEREINTERSPERSEDBETWEENNUCLEOTIDES889AND975WITHINORF2INBBSVXTHESETHREENUCLEOTIDEDIFEE佗NCESRESULTEDINAFRAMESHIFFDIFFEREOCEINCOMPARISONSWITHTHEBBSVNSTRAINTHATAFFECTEDAMINOAEIDS28510312OFTHEP82PROTEINWHICHMAYBETHEREPLICASEASSOCIATEDPROTEINTHEAMINOACIDSEQUENCEIDENTITYOFTHEP82PROTEINSOFBBSVXANDBBSVNWAS954％IALSOCONSTRUCTEDANINFECTIOUSCLONEOFBBSVXBYPLACINGTHECD_NAUNDERTHECONTROLOFTHE啊PROMOTERBASEDONTHEINEECTIOUSCDNASOFBBSVNANDBBSVXIEXCHANGEDPARTIALFRAGMENTSOFTHEP82GENEAFTERINOCULATINGCHENOPODIUMAMARANTICOLORANDVIGNASINENSISWITHINVITROTRANSCRIPTS，MTRANTSRESULTINGFROMTHEP92FRAGMENTEXCHANGESANDWILDTYPEVIRUSWEREOBSERVEDFORPHENOTYPICCHANGES，BUTTHESYMPTOMSANDHOSTRANGEWERENOTALTEREDTHUS，THEREAPPEARST0BENODIRECTREL撕ONSHIPBETWEENTHEVARIATIONOBSERVEDINP82ANDTHEPATHOGENICITYOFTHETWOBBSVISOLATESANINFECTIOUSELONEOFTHESATRNAOFBBSVXWASALSOCONSTRUCTED塒PLACINGTHECD_NAUNDERTHECONTROJOFTHE35SPROMOTERTHISPLASMIDCOULDINFECTCHENOPODIUMAMARANTO如RWITHTHEHELPOFINVITROTRANSCRIPTSOFBBSVXSIXDIF托RENTDELETIONANDINSERTIONMUTANTSOFBBSVXWERECONSTRUCTEDANDTHESEWEREINOCALATEDTOCHENOPODIWNAMARANTICOLOR11地PRELIMINARY船ULTSSHOWTHATTHESATRNACOULDENHANCESVMPTOMOFBBSVXANDITSJNSERTIONMUTANTSONCHENOPODIWNAMARAMICOLORBUTTHEPATHOGENICITYOFTHECPDELETIONMUTANTS蟠REDUCEDINORDORTODETERMINETHERELATIONSHIPBETWEENBBSVANDOTHERNECROVIRUSMEMBERS，INCLUDINGTHETYPESPECIESTOBACCONECROSISV0USTNV，IALSOSTUDIEDTHEBIOLOGICAL，PHYSICAL，CHEMICALANDSEROLOGICALPROPERTIESOFTHECHINASOYBEANISOLATEOFTNVR簿DATAP讒SENTEDBELOWINDICATE也缸THISISOLATEISANEWSTRAINOFTNVAWHICHIHAVED髓IGNATEDNⅣA。1K1NV小ISOLATEHASAHI曲STABILITYINVITROANDALSOHASABROADHOSTRANGEINCLUDING29SPECIESIN8FAMILIESTNVA。GENERALLYINDUEES10COLLESIONSINMOSTHO啦BUTSOYBEANANDⅣBENTHARAIANAALESYSTEMICHOSTSIPURIFIEDTHEVIRUSANDTHENOBTAINEDAHIGHTITER_NQVA。ANTISERUMTHERESULTSOFSEROLOGICALSTUDIESSHOWEDTHATTNVA。ISCLOSELYRELATEDTOTHETNVWILLOWISOLATEBUTFAILSTEACTWITH“INVDANDBBSVBASEDOBTNVSEQUENCESREPORTEDPREVIOUSLY，LDESIGNEDDEGENERATEPRIMERSANDOBTAINEDSUBCLONESREPRESENTINGPORTIONSOFTHETNVA。GENOMETHECOMPLETESEQUENCEOFTHEISOLATEWASDETERMINEDBY5“RACEAND3’RACETHEDATAREVEALTHATTHEVIRUSENCODESFIVEORFSINA3682NTGENOME，ANDTHATTHENUELEOTIDESEQUENCEIDENTITYISRESPECTIVELY864％437％AND443％BETWEENTHE1NVA。ISOLATEANDTHOSEOFTNVA，TNVDANDTNVD“WHOSESEQUENCESHAVEBEENREPORTEDPREVIOUSLYTHUSACCORDINGTOTHEBIOLOGICALPROPEAIESMADNUCTEOTIDESEQUENCESOFTHESESTRAINSIPOSTULATETHATTHESOYBEANISOLATEISANEWSTRAINOFTNVATOPRODUCEINFECTIOUSCDNACLONESTNVA。CDNAWASPLACEDUNDERTHECENTRELOFTHET7ANDTHE35SPROMOTERS，ANDDEIETIONMUTANTSINOR1，ERR3，ORF4ANDORF5WEREGENERATEDTHROUI曲ANALYZINGTHESYMPTOMSANDGENOMERNAREPLICATION，ICONCLUDETHATTHEORFIGENEISTHEREPLICASEASSOCIATEDGEREANDTHATORF3ANDORF4ARECELLTOEELLMOVEMENTGENESTHEPROTEINENCODEDBYORF5AFFECLSLONGDISTANCEVIRUSMOVEMENTWHICHRESULTSINATWODAYDELAYOFLESIONAPPEARANCEINCHENOPODIUMAMARANTICOLORKEYWORDSBEETBLACKSCORCHVIRUSPATHOGENICITYDIFFERENCE，TOBACCONECROSISVIRUBIOLOGICALPROPERTIES，INFECTIOUSEDNACLONE11

簡介：浙江大學碩士學位論文植物病毒侵染寄主的分子細胞學研究ⅠBBWV2在寄主體內(nèi)的復制和轉(zhuǎn)運機制探討ⅡSCMV和SRMV的細胞病理變化比較姓名王衛(wèi)兵申請學位級別碩士專業(yè)植物病理學指導教師洪健20040501SCMV引起風輪體、卷簡體和片層聚集體，屬于EDWARDSON等劃分的第1II型，一些成束的病毒粒子和內(nèi)含體存在于篩管中。SRMV引起風輪體和卷簡體，屬于第1型，一些內(nèi)含體分布于細胞壁附近，有的直接與細胞壁胞間連絲相接，完整的柱狀內(nèi)含體存在篩管中，推測內(nèi)含體在病毒的胞間運動中起定位和傳輸作用。兩種病毒在細胞病理學效應方面的差異也可以作為診斷鑒定的一個參考依據(jù)。關鍵詞蠶豆萎蔫病毒；甘蔗花葉病毒；高粱花葉病毒；細胞病理學；超微結構膠體金；內(nèi)含體管狀結構；電子顯微鏡

簡介：廣西大學碩士學位論文廣西豬繁殖與呼吸綜合征病毒的分子生物學研究姓名蔣小紅申請學位級別碩士專業(yè)預防獸醫(yī)學指導教師余克倫黃偉堅20040501廣西大學2004屈碩士畢業(yè)論文苷酸及其推定的氨基酸的同源性分別為938100％和952100％，與LV株的核苷酸及其推定的氨基酸的同源性分別為663％和639％。同源性分析顯示，GXA株與VR一2332、MLV和BD一4的同源性非常高，而GXA與LV同源性非常低。構建的系統(tǒng)發(fā)育樹分析，GXA與VR一2332、MLV及BJ4株親緣關系比較密切。從而表明GXA株屬于美洲型毒株，可能來源于疫苗株。將GXA株的E、M、N基因從重組質(zhì)粒PMDE、PMDM、PMDN中亞克隆至原核表達載體PET32A上，構建了原核表達重組質(zhì)粒PETE、PETM和PETN，并通過酶切鑒定，證實構建成功。關鍵詞豬繁殖與呼吸綜合征病毒PRRSV分離GXA株克隆測序同源性原核表達重組質(zhì)粒RESEARCHONMOLECULARBIOLOGYOFPORCINEREPRODUCTIVEANDRESPIRATORYSYNDROMEVIRUSPRRSVINGUANGXIABSTRCTINRECENT，SOMEPIGFARMSINGUANGXIOCCURFREQUENTLYANINFECTIOUSDISEASE，WHICHCHARACTERSAREABORTION，DEATHOFEMBRYO，MUMMIFIEDFETUSES，ANDRESPIRATORYPROBLEMSINPIGLETPATHOGENOFTHEDISEASEWASCONSIDEREDASPORCINEREPRODUCTIVEANDRESPIRATORYSYNDROMEVIRUSPRRSVINTHISSTUDY，WEIDENTIFIEDTHATTHESAMPLECOLLECTEDFROMTHEFARMINNARMINGWASPOSITIVEBYRTPCRWITHAPAIROFSPECIFICNGENEPRIMERSOFPRRSVBASEDONIT，PERMISSIVECELLS，MARC一145CELLSWEREINOCULATEDWITHTHESAMPLEAFTERSIXBLINDPASSAGES，TYPICALCYTOPATHOGENICEFFECTCPEWASOBSERVEDONCELLSASWELLASTHEPOSITIVECONTR01ANDPRRSVWASPOSITIVEINTHECELLSANDCULTUREMEDIUMBYRTPCRTHEFIRSTPRRSVSTRAININOUANGXIWASISOLATEDSUCCESSFULLY，NAMEDGXASTRAINANDITSINFECTIONTITERWAS105”TCID5N／M1

簡介：山東農(nóng)業(yè)大學碩士學位論文山東省H5N1和H9N2亞型豬流感病毒的分子流行病學調(diào)查姓名許傳田申請學位級別碩士專業(yè)預防獸醫(yī)指導教師趙宏坤范偉興20040526山東農(nóng)業(yè)大學碩L學位論文中文摘要近年來，山東省各地豬場不斷發(fā)生以呼吸道癥狀為主要表現(xiàn)的疾病，給養(yǎng)豬業(yè)造成了嚴重的損失，這些呼吸道疾病包括藍耳病、豬喘氣病、豬圓環(huán)病毒、豬流感等，有時為兩種或兩種以上的病原同時感染，為了弄清豬流感病毒在引起豬呼吸道疾病中所起的作用，20022003年我們在山東省20多個發(fā)病比較典型的豬場展開對豬流感病毒的分離鑒定和分子流行病學的調(diào)查。首先在各疑似豬流感發(fā)病地區(qū)進行采樣，按常規(guī)把病料處理之后接種SPF雞胚，在雞胚中進行連續(xù)傳代，每代無菌收獲雞胚液，用雞或豚鼠的紅細胞進行血凝和血凝抑制試驗來驗證是否有流感病毒，待傳代后雞胚液的血凝價較高并比較穩(wěn)定后再用標準血清進行定型。對初步確診為H5和H9兩個豬流感病毒株進行了電鏡觀察，并作了小自鼠攻毒和豬體回歸試驗來驗證這兩個毒株的致病性。對山東10個發(fā)病豬場的30份血清進行檢測，H5和H9的抗體檢出率為30％，盡管抗體水平不高由哈爾濱獸醫(yī)研究所流感中心代測，所測的血清中有H3和HL的豬流感病毒的抗體，但本次流感病毒分離中沒有分離到H3和H1亞型。在細胞上觀察H9和H5豬流感病毒引起的致細胞病變效應CPE，山東分離株H5和H9流感病毒能在雞成纖維細胞、HELA細胞，MDCK，以及MDBK上產(chǎn)生的CPE。分別挑選一株H9N2和H5N1亞型典型毒株進全基因克隆測序，利用DNASTAR軟件進行序列分析，并和GENBANK中的禽、人和豬的核苷酸同源性分析和氨基酸形成的進化樹進行分析，同時對HA的氨基酸裂解位點

簡介：V762‘5C奶牛持續(xù)性感染口蹄疫病毒的分子生物學檢測導師FII建民教疆“孔飪州究日Ⅱ，M覽膏Q基礎獸畦學0，0礎免＆學沈日衣_E上學攢簧大小約為838BP戇基因片段。DNA廖列分輯表甓，該部分基因與嗣態(tài)搬遂的0型FMD瘸海抹L冬基鞭瓣澡毪這88％。葒結聚為持續(xù)毪爨染F鬻蚤V靜分予浚幸亍瘸學誕壹提供了襖據(jù)。關鍵溺；爨蹲痰FMDV；逆轉(zhuǎn)豢聚會薅鑣式茨應；RNA搓鞭；滓歹分新

簡介：Y9500．70分類，J8526密級單位代碼1Q盟學號?S03，盟QQ8名微雇業(yè)大學學位論文安徽省豬圓環(huán)病毒病分子流行病學調(diào)查THEINVESTIGATEOFMOLECULAREPIDEMIOLOGYOFPCV2INANHUI研究生指導教師合作于}}導教師申請學位門類級別專業(yè)名稱研究方向所在學院揚世基魏建蠱副煎援塹疊羞熬援叢生塹』麴隨盞匡堂苤查籃鎏瘟堂塾塑型拉堂醫(yī)答辯委員會主席透鑫支又和DQL95679及AY556476、AY556473三個毒株關系較近，而與一株美國株AY325495、一株福建毒株AY556474共同組成的一個分支相距較遠。關鍵詞豬圓環(huán)病毒II型，分子流行病學，調(diào)查Ⅱ

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