粉蚧科昆蟲DNA條形碼鑒定技術及數據庫的建立.pdf

1、 華南農業(yè)大學 華南農業(yè)大學 學位論文原創(chuàng)性聲明 學位論文原創(chuàng)性聲明 本人鄭重聲明： 所呈交的學位論文是本人在導師的指導下獨立進行研究所取得的研究成果。 除了文中特別加以標注引用的內容外， 本論文不包含任何其他個人或集體已經發(fā)表或撰寫的作品成果。 對本文的研究做出重要貢獻的個人和集體， 均已在文中以明確方式標明。 本人完全意識到本聲明的法律結果由本人承擔。 作者簽名： 日期：

2、 學位論文版權使用授權書 學位論文版權使用授權書 本學位論文作者完全了解學校有關保留、使用學位論文的規(guī)定，即：研究生在校攻讀學位期間論文工作的知識產權單位屬華南農業(yè)大學。學校有權保存并向國家有關部門或機構送交論文的復印件和電子版，允許學位論文被查閱或在校園網上發(fā)布并供校內師生和與學校有共享協(xié)議的單位瀏覽（除在保密期內的涉密論文外） ；學校可以公布學位論文的全部或部分內容，可以允許采用影印、縮印或其它復制手段保存、匯編

3、學位論文。本人電子文檔的內容和紙質論文的內容相一致。 作者簽名： 日期： 導師簽名： 日期： 學位論文提交同意書 學位論文提交同意書 本學位論文符合國家和華南農業(yè)大學關于研究生學位論文的相關規(guī)定，達到學位授予要求，同意提交。 導師簽名：

4、 日期： 學科帶頭人簽名： 日期： II DNA Barcoding and Database Construction of Pseudococcidae Ren Jingmei (College of Agriculture, South China Agricultural University, Guangzhou

5、510642, China) Abstract: Mealybug ( Hemiptera: Pseudococcidae ) is a tiny insect group that feeds on plant sap. Its adaptability and the reproductive capacity is very powerful, and easily leads to disaster to agriculture

6、 and forestry areas. Mealybug is high alert pest of our country. With highly covert, it brought great difficulties to our entry quarantine. The rapid and accurate identification is the foundation of effectively monitorin

7、g and controlling to mealybugs. But there are many problems in the morphological indentification method. So looking for more efficient and convenient method of identification is particularly urgent. DNA barcoding is an i

8、dentification method that using sequence differences of molecular markers in species. In this study, we used the 5’ and 3’-end of COI gene to verify the effectiveness of identification in Mealybugs. We acquired 502 seque

9、nces of 5’-end and 356 sequences of 3’-end from 538 mealybug samples. The sequence length of them was 649bp and 820bp. The average contents of AT were significantly higher than GC for the COI sequences in mealybugs. The

10、intraspecific genetic distances range of 5’ and 3’-end were 0%~8.03% and 0%~9.5%. The interspecific genetic distances range of 5’ and 3’-end were 1.6%~22.89% and 1.99%~21.39%. Some overlap was observed in the distributio

11、n of genetic distances among conspecific individuals and interspecific individuals. The variation of different geographic populations in Ferrisia virgata and Phenacoccus madeirensis was very obvious, and had reached a le

12、vel of species. Phylogenetic tree of NJ showed that all species could form an independent clade. Phylogenetic tree of Pseudococcus, Phenacoccus, Phenacoccus and Dysmicoccus in NJ method showed that COI gene can distingui

13、sh the species of main genus in Pseudococcidae, and the result was basically consistent with morphological identification. The identification of closely related species was also effective. COI gene can be used for the ge