蟬擬青霉產(chǎn)透明質(zhì)酸的提取工藝及其性質(zhì)研究.pdf

1、貴州大學(xué)2009屆碩士研究生學(xué)位論文蟬擬青霉產(chǎn)透明質(zhì)酸的提取工藝及其性質(zhì)研究學(xué)科專業(yè)：生物化學(xué)與分子生物學(xué)研究方向：生物活性成分及分離技術(shù)導(dǎo)師：譚艾娟研究生：黃鍇中國﹒﹒﹒﹒貴州﹒﹒﹒﹒貴陽2009年6月分類號：Q539論文編號：2006221407密級：公開級貴州大學(xué)2009屆碩士學(xué)位論文蟬擬青霉產(chǎn)透明質(zhì)酸的分離純化及其性質(zhì)研究II3結(jié)果和分析..............................................

2、........................................................................253.1標(biāo)準(zhǔn)曲線的建立...........................................................................................................253.2透明質(zhì)酸發(fā)酵液處理方法研究...........

3、........................................................................263.2.1透明質(zhì)酸酶的失活條件研究..................................................................................263.2.2殘留菌體、蛋白質(zhì)去除方法研究..........................

4、.......................................................273.3透明質(zhì)酸粗品分離......................................................................................................31第三章蟬擬青霉產(chǎn)透明質(zhì)酸的純化工藝研究.............................

5、...................................321材料..................................................................................................................................321.1材料...........................................

6、...................................................................................322方法..................................................................................................................................32

7、2.1檢測方法......................................................................................................................322.2純化方法......................................................................................

8、................................332.2.1溴化十六烷基吡啶沉淀法.......................................................................................332.2.2離子交換層析純化法...................................................................

9、............................343結(jié)果與分析......................................................................................................................352.1標(biāo)準(zhǔn)曲線.........................................................

10、.............................................................353.2溴化十六烷基吡啶法與離子交換層析純化法對比..................................................353.3離子交換層析純化研究....................................................................

11、..........................363.3.1洗脫劑濃度的影響..................................................................................................363.3.2洗脫劑流速的影響..................................................................

12、................................383.3.3洗脫劑PH值的影響................................................................................................39第四章蟬擬青霉產(chǎn)透明質(zhì)酸的部分理化性質(zhì)研究.....................................................

13、...401材料..................................................................................................................................401.1材料...........................................................................

14、...................................................402方法..................................................................................................................................402.1透明質(zhì)酸的物理和化學(xué)性質(zhì).................

15、.....................................................................402.2透明質(zhì)酸純化樣品雜質(zhì)鑒定......................................................................................413結(jié)果和分析........................................

16、..............................................................................433.1透明質(zhì)酸的物理和化學(xué)性質(zhì)......................................................................................433.2透明質(zhì)酸雜質(zhì)測定..........................