普通小麥與羊草不對(duì)稱體細(xì)胞雜交的研究.pdf

1、山東大學(xué)碩士學(xué)位論文普通小麥與羊草不對(duì)稱體細(xì)胞雜交的研究姓名：陳秀玲申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：發(fā)育生物學(xué)指導(dǎo)教師：夏光敏2002.5.18山東大學(xué)碩士學(xué)位論文ABSlRACIProtoplastsofcommonwheat(TriticumaestivumL)CVJinan177originatedfromlongtermsubculturedcalliwerefusedwithprotoplastsofLeymuschinensissu

2、spensionculturessubcultruedfor5yearsusingPEGmethodTheprotoplastsofwheatwerenotcapableofregeneratingtocalliandwholeplantsTheprotoplastsofLchinensisalsohadlosttheabilitytodifferentiateBeforefusiontheprotoplastsof￡chinensis

3、irradiatedbyUVattheintensityof380IIW／cm2for30s，45s，IminrespectivelyThefusioncombinationT()L(UV30s)producedgreatamountofregeneratedclonescapableofsubsequentgrowthwhichwereidentifiedashybridsbymorphologicalandchromosomeobs

4、ervationaswellasisozymepauem(esterase，malicaciddehydrogenaseandperoxidase)andRAPDanalysisHoweverthe5S—rDNAspacesequenceanalysisshowednowheatcharacteristicbandsThereforetheaboveanalysisinawholedenotedclonethenatureofthehy

5、bridclonesandtheirnucleargenometendedto￡chinensisThefusioncombinationTf)L(UV45s)producedonlyseveralregeneratedcloneswhichcouldnotcontinuetogrowfurtherandturnedtobebrownanddiedgraduallyandincombinationT()L(UVlmin)noregene

6、ratedclonesweregainedForflpartofthehybridincombinationT()L(UV30s)，cytoplasmicgeneanalysiswerecarriedOUtHybridizationwithspecificprobeformitochondriagenome，mayorhybridcloneshadcharacteristicbandsofbothparentsandnewbands，m

7、inorofthemhadmerelycharacteristicbandsof￡chinensisandnewbandsUsingspecificproberbclforhybridizationwithchloroplastgenome，thehybridsshowedcharacteristicbandsofbothparent，andnewbandsSomeproblemswerediscussedinthispapersuch