逐漸干旱對牡丹實(shí)生苗生理指標(biāo)的影響和牡丹GA20-氧化酶基因的克隆與序列分析.pdf

1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文逐漸干旱對牡丹實(shí)生苗生理指標(biāo)的影響和牡丹GA20－氧化酶基因的克隆與序列分析姓名：逯久幸申請學(xué)位級別：碩士專業(yè)：園林植物與觀賞園藝指導(dǎo)教師：楊秋生201106逐漸干旱對牡丹實(shí)生苗生理指標(biāo)的影響和牡丹赤霉素合成相關(guān)基因GA20ox的克隆與序列分析EffectsofGraduallyDroughtonPhysiologicalIndecesofTreePeonySeedingandCloningwithSequenc

2、eAnalysisofGA20oxofTreePeonySupervisor：ProfYangQiushengMasterCandidate：LuJiuxingAbstract：SeedingsinPaeoniaostii‘FengDan’wereusedasmaterialtostudythechangesofphysiologicalindeces，suchaschlorophyllcontent，photosynthesis，CA

3、TactivityMeanwhile，takingPaeoniasuffruticosa‘Luoyanghong’asexperimentmaterials，thestudyclonedpartialfragmentsofGA20oxidasegene，whichishelpfulforobtainingthewholeeDNAandunderstandingtheroleofGAintreepeonygrowthanddevelopm

4、entThemainresultsareasfollows：1ChlorophyllcontentincreasedthendecreasedwiththeintensificationofdraughtIncontrasttonondrought，chlorophyllcontentmaximizedundermiledroughtincreasingby186％andminimizedunderseveredroughtdecrea

5、singby512％Pn，CiandGswerealldecliningandatthelowestvolumeunderseveredrought，seperatelygoingdownby88％，786％，894％comparedtonondroughtTrincreacedthendecreacedAfterpeakvolumeundermoderatedroughtTrdropedby50％underseveredroughtc

6、onparedtonon—droughtThechangesshowedthatphotosynthesisofseedingsWasrestrainedtosomedegreeCATactiviitychangesdeffirentlyinrootsandleavesInleaves，itrisedthendropedwimtheintensitificationofdraughtbutincreasedonthewholeunder

7、droughtstresscomparedtonondroughtInroots，CATactivitybroughtdograduallyandthestudyneedstobemorefurther2DesigningspecificprimersPSGA201andPSGA202accordingtothenucleotidesequenceofGA20一oxidaseofPaeoniasuffruticosa‘Luhehong’