質(zhì)譜法在茶葉分析中的研究.pdf

1、遼寧師范大學(xué)碩士學(xué)位論文質(zhì)譜法在茶葉分析中的研究姓名：吳寒申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：分析化學(xué)指導(dǎo)教師：侯冬巖；回瑞華201204質(zhì)譜法在茶葉分析中的研究TheresearchofteaanalysisinmassspectrometryAbstractIIlthispaperthechemicalcompositionsofteaarestudiedThecompositionsofoligomericproanthocyanidinsi

2、ntheteaareanalyzedandthecontentsofoligomericproanthocyanidinsandtheaflavinintheteaaredeterminedbyhighperformanceliquidchromatographyelectrosprayionizationtandemmassspectrometry(HPLCESIMSMS)Thecontentsoftraceelementsinthe

3、teaaredeterminedbyinductivelycoupledplasmamassspectrometry(ICPMS)UsingHPLC—ESI—MSMStechnique，thecomponentsofoligomericproanthocyanidinsintheteaarequalitativelyanalyzedbyfullscanandselectedionmonitoringmodeTheoligomericpr

4、oanthocyanidinsandtheaflavinintheteaarequantitativelydeterminedbyproductionSCanandmultiplereactionmonitoringmodeTheoligomericproanthocyanidinsandtheaflavinareextractedbyultrasonicmethodandthefactorsaffectingtheextraction

5、rateoftheoligomericproanthocyanidinsandtheaflavinaremadebysinglefactorexperimentandorthogonaltestThechromatographicconditions(suchasmobilephaseelutionproceduresandcolumntemperature，ere)andthemassspectrometryconditions(su

6、chasproductionandcollisionenergyetc)oftheoligomericproanthocyanidinsandtheaflavinareselectedandoptimizedInthedeterminationofoligomericproanthocyanidins，themethodofrecoveryrateisbetween914％to1098％andthecoefficientofvariat

7、ioniSlessthan75％Inthedeterminationoftheaflavinthemethodofrecoveryrateisbetween912％to1025％andthecoefficientofvariationislessthan41％Theaccuracyandprecisionoftwomethodsareveryhi曲nleoligomericproanthocyanidinsintheteaarequal

8、itativelyanalyzedbyapplyingHPLCESIMSMStechnology，threekindsofmonomers，threekindsofdimers(twokindsofnonestertypedimersandonedimericmonoester)andthreekindsoftrimersofoligomericproanthocyanidinsintheteaaremainlytestedThequa

9、ntitativeanalysisofoligomericproanthocyanidinsintheteashowsthatWuYiShanofRockTeaofShuiKuLingRouGuiFuJianTieGuanYinYunNanTangZhiZhenGuangDongBaXianGuoHaiTaiWanofOrientalBeautyinthecontentofProanthocyanidinB2inoolongTea；Ji

10、nJunMeiYinJunMeiChiGan(TongJunMei)inthecontentofProanthocyanidinB2inJunMeiseriesofBlackTeaThequantitativeanalysisoftheaflavininteashowsthat偉么YiShanofRockTeaofXianRenYanShuiXianTaiWanofOrientalBeautyGuangDongBaXianGuoHaiY

11、unNanTangZhiZheninthecontentoftheaflavininoolongTea；ChiGan(TongJunMei)YinJunMeiJinJunMeiinthecontentoftheaflavininJunMeiseriesofBlackTeaThus，theproanthocyanidinB2andtheaflavininoolongteaandblackteaareinverselyproportiona