P物質(zhì)及其受體NK-1在結(jié)腸癌中的表達及作用.pdf

1、青島大學碩士學位論文P物質(zhì)及其受體NK1在結(jié)腸癌中的表達及作用姓名：趙嶺嶺申請學位級別：碩士專業(yè)：病理與病理生理學（病理學）指導教師：陳樺20100529ExpressionandeffectofsubstancePandNK一1incoloncancerObsjeetObjective1TostudytheexpressionofsubstanceP(SP)anditsreceptor，neurokinin1(NK1)incolonc

2、arcinomasandhumancoloncancercelllineRKO2ToevaluatetheeffectofpotentselectiveNK1agonist【Sar9，Met(02)l1]substanceP(SMSP)andselectiveantagonistL733060onhumancoloncancercelllineRKOinvitro3Toinvestigatethepotentialfunctionofs

3、ubstancePandNK1inhumancoloncancercelllineRKOMethods1TheimmunohistochemistrystudywasperformedtodetecttheimmtmolocationofsubstancePandneurokinin一1incoloncarcinomas(n=85)Severalspecimenswerealsoselectedtostudyasfollows：norm

4、alcolonspecimens(n=l3)，adjacentspecimens(n=22)hyperplasticpolypsspecimens(n=26)，adenomasspecimens(n229)2WecarriedoutaninvitroimmunocytochemicalstudytoinvestigatethelocalizationofsubstancePandNK1inRKOcells3variOUSconcentr

5、ationsofSMSP(10。10M一10擊M)andL733，060(109M10’5M)were_appliedaloneinRKOcellsMTTassaywereusedtodetectthecytoactivationResults①WeshowherethatcoloncarcinomacouldCO—expressbothsubstancePandNK一1TheimmunopositivesubstancePcellsw

6、eredistributeddiffuselyandthemajorityofSPlabelingWaslocalizedwithinthecytoplasmaofcoloncarcinoma，showedadiffuseornest—likecytoplasmicstainingHoweverNKlcouldbeobservedinbothplasmamembraneand／orcytoplasma②SubstancePandrece

7、ptorneurokinin1werealsodistributedinthecytoplasminnormalcolorectalmucosalspecimens，hyperplasticpolyp，colonadenomaandadjacentspecimensoncytoplasm⑨InRKOcells，wefoundimmunoreactivesubstancePwasmainlypresentedoncytoplasmandN

8、K1onbothmembraneandplasma④Ataconcentrationof10。8MSMSPexertedmaximumproliferationactionandL733，060(10m10擊M)inhibitedcellgrowthinadosedependentmannerConclusion1GiventhatbomcoloncarcinomatissuesandRKOcelllinebearoverexpress

9、ionofsubstancePandNK一1，itissuggestedthatneuroendocrinemechanismsparticipatethedevelopmentofcoloncarcinoma2L733，060couldbluntcellgrowthviaNK一1invitro3SubstancePmaystimulatecellproliferationthroughNK—1invivo；NK1mayserveasa