葡萄糖神經(jīng)酰胺合成酶在人白血病細(xì)胞中的表達(dá)及其與多藥耐藥的關(guān)系.pdf

1、y956563鐘犬碩士學(xué)位論文(2006屆)葡萄糖神經(jīng)酰胺合成酶在人白血病細(xì)胞中的表達(dá)及其與多藥耐藥的關(guān)系ExpressionofGiucosylceramideSynthasemRNAinHumanLeukemiaCellandItsRelationtoMultidrugResistance研究生姓名葛盍接指導(dǎo)教師姓名煎遺互墮專業(yè)名稱疸壟堂量疸壟生墨鱟研究方向盟瘤的生病扭劍論文提交日期2QQ生目葡萄糖神經(jīng)酰胺合成酶在人白血病細(xì)胞中的表

2、達(dá)及其與多藥耐藥的關(guān)系英文摘要ExpressionofGlucosylceramideSynthasemRNAinHumanLeukemiaCellandItsRelationtoMultidrugResistanceAbstractobjectiveToinvestigatethedifferenceofglucosylceramidesynthase(GCS)mRNAexpressionbetweendrugsensitiveand

3、drug—resistanthumanleukemiaTostudytherelationshipbetweenGCSandmultidrugresistanceinhumanleukemiaandexplorethemolecularmechanismofmultidrugresistanceinducedbyGCSMethodsTheclinical—dignosedleukemiaperipheralbloodsampleswer

4、ecollectedAfterseparatedtheplasmathemonocyteswereseparatedbyFicollandtotalRNAwasabstractedWedesignedtheprimersaccordingasthesequenceofGCS、mdrl、13actin、bax、bcl2geneinGenbankSemiquantitativereversetranscriptional—polymeras

5、echainreaction(RTPCR)WasemployedtoanalyzetheexpressionofGCS，mdrl，bcl一2andbaxmRNAindrug—sensitiveanddrug—resistanthumanleukemiaTherelativitybetweenGCSandmdrl，andbetweenGCSandbcl一2WasanalyzedseparatelyInadditionallyELISAwa

6、susedtodetecttheexpressionofbcl一2proteinfromtheplasmaofsamplesResults1ThecomparativeopticaldensityratiosoftheamplifiedGCSstrapsinthedrug—resistantgrouparesignificantlyhigherthanthedrug—sensitivegroup(P005)2ThereWasthepos

7、itivecorrelationboththeexpressionofGCSgeneandmdrlgeneinleukemiasamples(P001，Y=O66)3Thecomparativeopticaldensityratiosoftheamplifiedbcl2strapsinthedrugresistantgrouparesignificantlyhigherthanthedrug—sensitivegroup(P005)，T

8、heratioofbcl一2／baxinthedrug—resistantgroupWashigherthanthedrug—sensitivegroup(P005)4TherewasthepositivelinearcorrelationboththeexpressionofGCSgeneandbcl一2geneinleukemiasamples(P0，01，Y2O69)5ELISAresultsshowedthebcl一2prote