化學(xué)合成聯(lián)酶法生產(chǎn)D-對羥基苯甘氨酸的研究.pdf

1、天津大學(xué)碩士學(xué)位論文化學(xué)合成聯(lián)酶法生產(chǎn)D對羥基苯甘氨酸的研究姓名：崔哲峰申請學(xué)位級別：碩士專業(yè)：制藥工程指導(dǎo)教師：劉明言付瑞良20031201AbstractDP，hydmxyphenylglycine∞P—HPG)isonekindofimportantintermediateforthepreparationofsemisyntheticantibioticssuchassemisyntheticamoxicillin、ceforp

2、erazoneandcefoprozilInChina，thesatisfactionofneedonDp—HPGdependsonimportation。SoitisnecessarytostudytheproductionprocessesofDPHPGtofindanewproductionmethodthatiseconomicalandhighefficianLThechoiceandbreedingoffungusiskey

3、factorinproductionofDPHPGbyenzymemethod。InthispaperthePseudomonasstriata￡2399whichcanproduceDhydantoinase(DHase)andDNcarbamylase(D—Case)waschosenThesetwokindsofenzymecandirectlytransformDphyxlroxyphen薅hydantoin(DPHPH)toD

4、pHPGByexperiments，thefermentationconditionsoffunguswereoptimizedandtheoptimaltemperatureandpH萌豫筠determinedTheenzymaticreactionCOnditionswerealsooptimizedbyexpedmentsandtheoptimalreactiontemperatureandpHwerealsodetermined

5、。Throughoptimizationtheyieldrateofenzymaticreactioncanreachto99％thatisnolowerthantheJapanproductiontechnologyThemechanismofenzymaficreactionWaSfoundbyanalyzingthetransformationprocessesT№abstractionprocessesofD—pHPGweres

6、tudiedBycombinationofenzymecatalysisandchemistrysynthesismethod，theseparationofproductfrommotherliquorwassuccessfullyachievedtnordertogetproductwithhi癆qualitythesecondtimeresinandmixedbedsexchangetechniqueswereused，andth

7、etraditionallX7resinweregivenupTroughthismethod。thequalityofproductisbetterthanproductimportedinsomewaysThenewtechnologywassuccessfullyindustrializationinShijiazhuangZhongtianChemicalIndustryCoLtdTroughtestinindustrialma

8、nufactureitWaSfoundthatthequalityofproductisasgoodasthoseimportedorbetterTheproductioncostofthenewmethodislowerthansimplechemistrysynthesismethodandenzymecatalysismethodTheyieldrateofthismethod(calculatedfromDpHPH)Callre