大鼠蛛網(wǎng)膜下腔出血后骨髓基質(zhì)細(xì)胞源性神經(jīng)干細(xì)胞移植后的修復(fù)作用.pdf

1、青島大學(xué)碩士學(xué)位論文大鼠蛛網(wǎng)膜下腔出血后骨髓基質(zhì)細(xì)胞源性神經(jīng)干細(xì)胞移植后的修復(fù)作用姓名：王金偉申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：神經(jīng)外科指導(dǎo)教師：姚維成20070526英文摘要Theitpairiageffectoftransplantationofbonemrrowstemcellsaftermbarachnoidhemorrhage缸adultratsAbsh：礎(chǔ)Objective：Brainedemaandseriomuenrondamag

2、emaytakeplacethroughdifferentmechanismsresultingfromcerebralvi悖∞pi啪andintracranialhypertensionafter缸嘲Iri鋤alsubarachnoidhemorrhage(SAH)whichleadtolackofneurologicalfunctionofthepatientsandtheorthodoxtherapyis嘲Dlc∞foritNeu

3、ralstemcellstransphntationhasraisedhopesthatresearchers鋤findwaystorepairflefvoussystemdamageBonen姐【n鉀sUomal洮(SMSCs)canbetheideal$otulzfortransplantationduetotheirstrongabilitiestogelleWanddifferentiateandtheirabundanceso

4、urcewithouteitherethicalworriesorpotentialimmunologyfejectionTheobjectiveofthispilotlestjstostudytherepairingeffectoftransplantationofbonema嗍UromalcellsderivedneuralstemeeIIs(BMSCsDNSCs)offadultWmtarratswithSAHandprovide

5、mo∞theorysupportforneuralstcmcellstransplantationMetheds：Bonenl釧嚏owcellsw∽collectedfmmthefemurand缸biaofadultwistarmts1kmBMSClwegeharvestedbyINgangofdemitygradientcentfifugation。BMSCifromadultwistarratswmculturedwithneura

6、basalmediumandEOFbFGElmmunocytochemistrywasperformedtoidentifythedifferentiatedcellsBh略CsDNSCswe他labeledwith5Bmmo2’deoxyundme(BrdU)45adultVnmtarratswc∞r(nóng)andomlym、，idcdintothethreegroupsofcontrol、DM]BM(DulbeccosModifiedEag

7、bMedknn’andBMscsDNSCa脅bemgsuccessfullymadeintothemodelsof鞘1Im鼢Hmodelswereinducedby蜥ectionof”mIautologousbloodintothee1sternamagnatwice24hourslatertheDMEMgroupandBMscs’DNsCsgroupwe∞r(nóng)espectivelyinducedby硒ectionof30IIlⅨ壓Man

8、d30ul∞II(38x10噸MSCsDNSCs)suspensionintothecisternamagnaofthes^HmodelsThc地mol蟬面alfanet／omal舯de蹴惴usedtoevaluatetheneuralfwlctioaofrats砒di位rentintervalsfo[[owingthetransplantationTheBrdUpositivecellswcledetectedwimimmunohis

9、tochemicalslainingmethodResultsAt5dafterSAntherewas110statisticaldi疵fen∞inneurologicalfunctionalgm&s∞∞ofthethreegtoups(F=O075PO05)JulOd、20d、30dand40dafarsAHthedifferencebetweenthe＆懾groupandtheD硼B(yǎng)MgroupwashⅨ謗Ii?！辬t(1嘲沿5)，

10、butcomparedwitheitherofothergroups，BMSCsDNScsgroupwasobservedameliorationintheneuralfunction(PO05)：1kB刪pmitive∞llsweredetectedinthebrainsoftheratsConclusion：TransplantationofBMSCsDNSCscaneffectuallyimprovetheneuralfuncti

11、onofratswithS^H：ThenewgeneratedneuronsdifferentiatedfromBMSCs、DNSCSmayexerta∞cpaidnginfluenceonthebrainafterSAILPostgraduatestudent：WangJinwei(Neurosurgery)DirectedbyProf：Yao_eichengKeywords：subarachnoidhemorrhage：bonems