紫蘇光周期反應(yīng)與類LFY基因cDNA的克隆.pdf

1、海南大學(xué)碩士學(xué)位論文紫蘇光周期反應(yīng)與類LFY基因cDNA的克隆姓名：蘭樹斌申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：李建國20080601紫蘇光周期反應(yīng)與類即甲基因cDNA的克隆AbstractPemla(P洲婦夕甜幻scP珊L)isatypicalshortdayplantPhotoperiodinnuences恤gr0、砒anddeVelopmentofP鰣lla，itisdecisivet0regulatetheeXpress

2、ionofgenesrelatedt0floraJdeVelopmemT11isp印erstIldiednlee插：ctofphotoperiodto也e黟owtllanddeVelopmentofPerillaApiVotalge北丘agmentrelated、V弱clonedalldexpressediIlthenoraJdeVelopment1Efj陪ctofsupplementarylightphotop耐oda芏1dlumjn

3、os塒ongrowdlanddevel叩mentofPerillaw觴had咖diedbysuppIementar)，light锨斌mentatIlight碰ertlleseedlingcameoutTheresultshowst11att王lenorescencelater，leafnuInberandplamheightincreaSing、現(xiàn)thsupplemen哪li出lurninos時(shí)洫creas證gdu血g3000luX；T

4、llelon薩rsuppl鋤ent哪ligbtphotoperiodismelaternowerbuddi疏rentiationdWing6hThemoresllSceptiVeofsupplemen乜町lightofP舐11a，themoredistinctofdelay—e毹ctoffloweringinlongsupplementa巧light1tw嬲眥ggestedmatlowsupplementa巧lightluminosit

5、yor10hsunlightsuitf犯tpropagation，and3000lu)【supplementa】哆lightluminos塒and6hsupplementar)，lightphotoperiod，e嬲yt0maIlipulatebynuorescentl鋤p，bemostsui訕let0Vegetative孕。謝h，whichcallincreaSedyieIdaIldsaVeene瑪y嬲well2Accordingto

6、t11eresultSofabove也esnldyPemlasweregr0啪in觚odi船rentcomrolledphotoperiodconditioIlS謝廿lsⅢ11ightof10haIldl6h，respectiVelyt0obsenretheinfluenceofphotop嘶odt0鯽伽l雒ddevelopmentinP耐llaTheresults艄icatedthatcompared嘶血shonphotoperiod

7、，uIlder10ngphotop舐od，pl趾theight，caudex，leafandkafmⅡnberincteaSed，florescencedek畔dThesecharactIerscanbeusedaScriteI協(xié)ntoex鋤inephotoperiodsensitiVit)，ofPeriUa3’IhephaSe硒mtlecalyxdi毹rentiationstaget0gylloeciadif詫rentiationst

8、agelaSted34daysisnowerbuddi行erentiationofPe礎(chǔ)aiIlthemicroscopeA445bpcDNA∞脬lil【e)療agmentwaSclonedbyRTPCRandmmedpLFY，usingplantmaterialof“sphaSeSequencea11羽ysisshowedmatmecDNA觸舯enthadhomologyof90％晰tll凡DJ，67％晰tllB㈣9l％謝t11凡0嘣

9、2，67％晰tll￡n，，80％嘶tll兕DDesi弘ingmePCRp灑ersusing也ecDNA螄弘entsequenceofPerillaA308bpcDNA觸鯽entwaSclonedby37RACEAnewcDNA觸gmentsequenceof、^，asmadeupoftllistw0sequencesTheresultofBlaS欽sho、張dmatmeSplicedfhgmentsharedaKghde鏟eeofseq