腫瘤微環(huán)境對(duì)腫瘤相關(guān)巨噬細(xì)胞形成的影響及其機(jī)理探究.pdf

1、中山大學(xué)碩士學(xué)位論文腫瘤微環(huán)境對(duì)腫瘤相關(guān)巨噬細(xì)胞形成的影響及其機(jī)理探究姓名：陳旎妮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：細(xì)胞生物學(xué)指導(dǎo)教師：鄭利民20070608生坐奎蘭堡主蘭堡堡苧壁塑塑堡堡翌壁塑塑莖曼堂塑些絲壁塑墅墮壟苧墊翌簍塑Tumormicroenvironmentsinduceformationofimmunosuppressivephenotypeonmacrophages_一thefundamentalstudyonmechanismM

2、ajor：CellBiologyName：NiniChenSupervisor：ProfLiminZhcngAbstractEvidencesfromclinicalandexperimentalstudiesindicatethatmacrophagcsinsolidtumorsareunabletostimulateeffectiveanti4umorilDIllUIIeresponses；onthecontrary，theyexh

3、ibitadistinctimmunosuppressivephenotype，whichfacilitatetumorprogressionandmetastasisTheshiftofmaerophages丘omimmunestimulatingtoinamui七suppressingphcnotypcisdeterminedbythetumormicroenvironmentsButthemechanismsonhowtumorm

4、icroenvironments“educate“macrophagesarenotwellunderstoodTheexperimentsshowedthatculturesupernatantfrsh0derivedfromtwotypesofsolidtumorcelllinesU251and95DcoulddrivemonocytestobecomeimmunosuppressivemacrophagesKineticexper

5、imentsrevealedthat，soonaiderinitialexposuretothoseTSNmonocytesbegantoprovoketransientproinflammatoryresponsesWhereaflertheyextinguishedtheirsynthesisofproinflammatorycytokinesandbecameinactivetosubsequentstimulationTSNli

6、omUquidtuinorcelllineTI1P1failedtotriggersuchtemporarypreaetivationanddidnotaltermacrophagespolarizationMoreovertheintermediate—sizeHAhyaiuronanfragments(INTHA)wasfoundtohavethesameeffectsasTSNofU251and95DORhumanmonocyte

7、sandmacrophagesTheseresultsindicatethatsolublefactorsderivedfromsolidtumorsincludinghyahrormfragmentsremodeledthenormaldevelopmentofrecruitedmonocyte5frombloodbyeducatingthemthroughdifferentnichesoftlH蹴Themalignantcellsc