青紫薯色素對自然衰老大鼠體內(nèi)氧化水平和Aβ誘導(dǎo)神經(jīng)細胞毒性的作用研究.pdf

1、AIM to investigate the effect of sweet-potato anthocyanins on oxidation conditions in aging mice and cytotoxicity of PC- 12 cells induced by Amyloid β protein (Aβ). METHODS we use the natural aging Wistar rats as experim

2、ental models. The animals were randomly divided into six groups, including normal young control group, aging model group, low concentration, middle concentration and high concentration sweet-potato anthocyanins groups an

3、d vitmin E as positive control group. We measured the total antioxidative capacity (T-AOC), activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), reactive oxygen species (ROS) and malondialdehyde (MD

4、A) of different organs from the animals. And we also established the cytotoxicity of PC-12 cell model induced by Aβ. By using cell culture, MTT assays, flow cytometry, we measured the cell viability, apoptosis, activity

5、of caspase-3, and mitochondria membrane potential, intracellular Ca2+ in PC12 cells. RESULTS sweet-potato anthocyanins can increase the activities of T-AOC, SOD and GSH-px and decrease the amount of ROS and MDA in severa

6、l organs from the animal in a dose-dependent manner (p＜0.05, p＜0.01). MTT assays showed that the PC-12 cell viability decreased under A β treatment. Sweet-potato anthocyanins can increase the PC-12 cell viability, stabil

7、ize the mitochondria membrane potential, and decrease the concentration of intracellular Ca2+ dose dependently (p＜0.05, p＜0.01). CONCLUSION sweet-potato anthocyanins modulate the oxidation condition of aging rats through

8、 its ability of compensating the decline in T-AOC and the activities of antioxidant enzymes and thereby inhibit the aging process. Also sweet-potato anthocyanins can protect the PC-12 cell from A β induced injury. Thus i