急性白血病GST-π與LRP基因及編碼蛋白的臨床研究與檢測.pdf

1、內(nèi)蒙古醫(yī)學(xué)院碩士學(xué)位論文急性白血病GSTπ與LRP基因及編碼蛋白的臨床研究與檢測姓名：王靜申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)指導(dǎo)教師：肖鎮(zhèn)20090501TheinvestigationandexaminationofGST—qf、LRPgeneandtheirrelatedproteinsinacuteleukemiaAbstractObjectiveMultidrngresistancecaneventuallyresultinthec

2、hemotherapyfailtureofacuteleukemiaThroughexaminationofGST一面geneandlungresistance—relatedprotein(U心)geneandtheirtelatedproteins，80舾toshow：therelationshipbetweenthemandthemulti—drugresistanceofacuteleukemiapatient；thecorre

3、lationbetweenGST一仃andLRP；therelationshipbetweenthemandclinicaltypeofacuteleukemia；therelationshipbetweenthemandWBCcountMethodsRealtimefluorescentquantiWlvereversetranscriptionpolyme瑚echainreaction(RQPCR)W88usedtodetermin

4、eGST一1randLRPgeneinperipheralbloodmononuclearcellof44ALpatientsand27normalsubjectsAndS—Pimmuno—histochemicalstainingmethodwasusedtoexaminateGST一訂andLRPproteinin40ALpatientsand20normalsubjectsANOV，ttest，linecorrelationana

5、lysis，X2testwereusedResultsExpressionofGST一1rorLRPgenehad110significantdifferenceindifferentWBC(white—blood—cell)countgroupanddifferentclinicaltypegroup(acutelymphohlasticleukemia，ALL；acutenonlymphoblasficleukemia，AmJ)Co

6、mparisionofGsr一霄geneexpressionWassignificantdifferentbetweenprimary@xmpandcompleteremissiongroup，refractorygroupandcompleteremissiongroup(PO05)ThedifferenceofGST一1rproteinexpressioninrefractorygroupandcompleteremissiongr

7、oupw酗significantdifferent(P001)The8Rme嬲LRPproteinConclusionWBCcountandthetypeofleukemiashouldntinfluencethe既pressionofGST一，TrandLRPgeneOverexpressionofGST一霄andLRPgeneindicatepeerprognosisTheywererelatedwithcHnicalresista

8、nceThemuhidrugresistantmechanismofGST一1randLRPgeneisdifferentCo—examinationofGSTqrandLRPgeneprobablyhadgreatersignificancethanexaminationoforlekindofgeneinp陀擊c血gtheprognosisofacuteleukemiapatientsTheresultsofGSrr一宵andLRP