幾種CKIs在桂林地區(qū)常見惡性腫瘤中變異的研究.pdf

1、同濟(jì)醫(yī)科大學(xué)碩士學(xué)位論文幾種CKIs在桂林地區(qū)常見惡性腫瘤中變異的研究姓名：黃健申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：屈伸19990501AIJERNATlONOFTHREECKlsINSOMEMALIGNANTTUMoURSAbstractP16andP21geneweredetectedbymutiplex—PCRandtheexpressionofP16、P21andP27wereobservedbyinunohi

2、stochemicalassayin318tissuesof22tumortypestoundersiandthealternationoftheseCKIs(Cyclindependentkinaseinhibitor)inrealignanttumorsofourareaTheresu]tsofthestudyshowedthedeletionrateofP16genewas377％(120／318)，andtheloserateo

3、ftheexpressionofP16geneswas353％(66／187)NodeletionofP21genewasdetectedTheloserateoftheexpressionofP21genewas364％(68／187)，andthatofP27‘genewas412％(77／187)Thetotalloserateofexpressionofthethreegeneswas668％(125／187)TheCodele

4、tionrateofthetwoCKIswas466％(57／125)andthatofthethreeCKlswas16％(20／125)TheresultsofthestudyalSOshowedthatthedeletionofP16genewasrelatedtOhistologicaltypingandgradingofthetumorsandthemetabasiSoflymphnodeSoiStheP21geneTheex

5、pressionofP27intumorcellswasa1ittleweakthanthatinnormolcellsIthasbeensuggestedfromourstudythatthethreegenesmayhaveanimportantroleinthedeve]opmentoftumorsInnormaltheysuppresscellatGlphasetegulatecellcycleincaseofthattheab

6、normalcellescapefromthesupervisionofthenormalcellcycleC1ihicalapplyofthethreeCKIswillprovidesupplementaryindexfortumorsbygenediagnosis、distinguishmentandprejudgmentItalsoofferssomeexperimentfoundationsforthefurtherstduyo