左旋棉酚對人纖維肉瘤HT1080細胞凋亡作用.pdf

1、新疆醫(yī)科大學碩士學位論文左旋棉酚對人纖維肉瘤HT1080細胞凋亡作用姓名：劉朝政申請學位級別：碩士專業(yè)：外科學（骨外科）指導教師：白靖平2011-04新疆醫(yī)科大學碩士學位論文 新疆醫(yī)科大學碩士學位論文 2 2 The effect of apoptosis of L-Gossypol on human fibrosarcoma HT1080 cells and its underlying mechanism Postgraduate

2、:Liu chaozheng Supervisor: Prof.Bai jingping Abstract Objective: To investigate the anticancer activities of L-Gossypol on human fibrosarcoma HT1080 cell proliferation and the induction of the molecular mechanisms of

3、 tumor cell apoptosis. Methods: In this study, using human fibrosarcoma cell line HT1080 cells in vitro, observing the effect of L-gossypol on cell growth. The assay of MTT on HT1080 cell is to measure the proliferation;

4、 Hoechst33258 and the electron microscopy after using L-gossypol is to observe the morphological changes of apoptosis in HT1080 cells; flow cytometry assay is to measure the early apoptosis rate; colorimetry reflect the

5、activity of caspase-9 ,3 changes before and after using L-gossypol; Western blot measure the levels of Bcl-2, Bax protein after using L-gossypol.Results: L-gossypol inhibit the proliferation of HT1080 human fibrosarcom

6、a cells, and this inhibition depends on the working time and dose, the effect of L-gossypol is increasing by the increasing of the time and dose . HT1080 cells change the morphologic after using L-gossypol, Hoechst33258

7、reflect the high chromatin staining after using L-gossypol, electron microscopy show the typical morphological changes of apoptosis after using L-gossypol.The flow cytometry detect the effect of inducing early apoptosis

8、on HT1080 cells by L-gossypol. In the process of L-gossypol inducing apoptosis in HT1080 cells, there are changing of Cacspase-9 and 3 activation. Anti-apoptotic protein Bcl-2 expression is decreasing and the expression

9、 of Bax is increasing depend on the increasing of concentration of L-gossypol.Conclusion: L-gossypol can inhibit the proliferation of HT1080 human fibrosarcoma cells and promote their apoptosis. L-gossypol may has the ef

10、fect of inducing of apoptosis by increasing the caspase 9, caspase-3 activation, decreasing Bcl-2 protein expression, increasing expression of Bax protein in the regulation of play at the molecular level the role of apop