棉酚聯(lián)合順鉑對宮頸癌Hela細胞增殖與凋亡的影響.pdf

1、石河子大學碩士學位論文棉酚聯(lián)合順鉑對宮頸癌Hela細胞增殖與凋亡的影響姓名：王于理申請學位級別：碩士專業(yè)：腫瘤學指導教師：鞏平20090601AbstractObiectives：Traditionallyradiochemotherapyhasbeenthechoiceoftreatmentforcervicalcarcinoma；howeveritalsoproducesserioussideeffectssuchashemorrh

2、ageandinfectionThesideeffectsdramaticallyimpactpatients’qualityoflifeanddiscouragelongtermuseoftherapyThereis，consequentlyacriticalneedfornovel，moreeffectivedrugswithfewersideeffectsincervicalcarcinomatherapyGossypol，ana

3、turalcontraceptivedrugwithfewersideeffects，hasrecentlybeenproposedtobeapotentdrugtotreatsomeneoplasmsToinvestigatetheeffectofgossypolandgossypolbasedcombinationDDPonHelacellsofcervicalcarcinomaMethods：Thesensitivitytesto

4、fgossypoi、DDPandgossypolcombinationwithDDPWascardedoutbyMTTassaytoobservethecellgrowthinhibitingrate；CellapoptosisWasobservedbyfluorescencemicroscopy—acridineomnge／ethidiumbromidedoublestainingandflowcytometryCellprotein

5、ofBcl2andBclxLwasobservedbyimmunohistochemistryResu1t：TheresultofMTTassayindicatedgossypolwaseffectiveininhibitingtheproliferationofHelacellsTheincreasingeffecttimeandconcentrationofgossypoltesultintheincreasinginhibitio

6、nrateTheinhibitingmtesofDDPtoHelacellswereenhancedsignificantlybythecombinationwithgossyp01TheresultofAO／EBdoublestainingandflowcytometryshowedthatgossypolandDDPaloneorincombinationinducedHelacellsapoptosissignificantlyT

7、heeffectofinducingapoptosisofgossypolandDDPincombinationWaSsignificantlymorethanthemaloneTheresultofimmunohistochemistryindicatedthatgossypolandDDPaloneorincombinationdownregulatedexpressionofBcl一2andBclxLTheeffectofdown

8、regulatingexpressiontoBcl2andBclxLDDPwereenhancedsignificantlybythecombinationwithgossyp01Conclusion：GossypoleffectivelyinhibitstheHelacellsofcervicalcarcinomaandinducesitsapoptosisThesensitivityCanbeenhancedbythecombina