臨產(chǎn)前后妊娠組織中核因子-κB的p65蛋白表達及其與IL-8、TNF-α的相關(guān)性研究.pdf

1、遵義醫(yī)學院碩士學位論文臨產(chǎn)前后妊娠組織中核因子κB的p65蛋白表達及其與IL8、TNFα的相關(guān)性研究姓名：羅克燕申請學位級別：碩士專業(yè)：婦產(chǎn)科學指導教師：王豫黔20050501臨產(chǎn)前后妊娠組織中核因子KB的p65蛋白表達及其與IL8、TNFa的相關(guān)性研究Astudyontheexpressionofthep65anditscorrelationwithIL8、TNFabeforeandafterlabor[Abstract]Object

2、：(1)Thepurposeistostudytheexpressionofp65，IL8andTNFainthepregnancytissuesandthelevelsofIL8andTNF—aintheamnioticfluidandthematernalsen1nlbeforeandafterlabor(2)Thecorrelationbetweenp65andIL8orTNFaisbeeninvestigatedrespecti

3、velyduringthecourseoflaborMethods：SixtywomenattermpregnancyweredividedintotwogroupsaccordingtowhethertheywereinlaborornotBothgroupswerethirtywomenandtheywerealldeliveredbycesareansectionSpecimensincludingplacenta，decidua

4、s，myometriumandamnioticfluidwereobtainedduringthecourseofcesareansectionMaternalbloodswereobtainedbeforecesareanExpressionofp65，IL8andTNFaonplacenta，deciduas，andmyometriumweredetectedbyimmunohistochemicalSABCmethodandcom

5、puterquantitativeimageanalysisThelevelsofIL8andTNFaweremeasuredbyradioimmunoassayResults：(1)p65，IL8andTNFawereexpressedplacenta，deciduas，andmyometriumofbothgroupsintermTheproteinexpressionofp65，TNF—aonplacenta，deciduaswe

6、resignificantlyhi曲erinthegroupoflaboronsetthanthegroupnotlaboronset，buttheexpressionofIL8wasnotstatisticallydifferenceinthetwogroupsTheproteinexpressionofp65onmyometriumwassignificantlyhigherinthegroupoflaboronsetthanthe

7、groupnotlaboronset，buttheexpressionofTNFawerenot(2)ThelevelsofIL8andTNFawereallsignificantlyhigherintheamnioticfluidaftertheonsetoflaborTheleveIsofTNFainthematernalSelqlmhadtlosignifieantlydifferencebeforeorafterlaborpai

8、n，butthelevelsofIL8inthematernalserumwasnotdetectinthetwogroupsTherewassignificantlypositivecorrelationsbetweentheexpressionofp65ondeciduas，myometriumandthelevelsofIL8andTNF。ainamnioticfluidConclusion：Theexpressionofp65I