腫瘤表達(dá)譜基因芯片篩選膽囊癌腫瘤相關(guān)基因的研究.pdf

1、浙江大學(xué)博士學(xué)位論文腫瘤表達(dá)譜基因芯片篩選膽囊癌腫瘤相關(guān)基因的研究姓名：馮雪冬申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：外科學(xué)（普通外科學(xué)）指導(dǎo)教師：彭淑牖20050401博士季隹靜喜晰江土拿正學(xué)院馮量冬ABSTRACTobjective：TofindoutthedifferentialexpressiongenesingallbladdercarcinomabyprofilingcDNAmicroarrayandtOprovidedataforthed

2、iagnosticeDNAmicroarrayThevalidationofthemicroarcaywasconfirmedsimultaneouslyMethods：ThefreshspecimensofgallbladderandnormalcontrolwerepreservedinCryopreservation，thenthetotalRNAwaSextractedanddetectedHybridizationwithBi

3、ostarH40stypeprofilingeDNAmicroarraywasperformedaftertheRNAwaslabeledwithfluorescentprobeTheimagescanmnganddataanalysiswereperformedsubsequentlyFinallytheresultsofhybridizationwerevalidatedbyreal—timePCRResults：61differe

4、ntialexpressiongenesweledefinitedinaLl4specimens，whichwasinvolvedinseveralaspectoftumordevelopmentTherewere17genesdown—ragulatedand44genesupragulatedTherewere9oncogeneandanti—oncogene；7genesaboutapoptosis；6genesaboutSynt

5、hesis，repairandreconstructionofDNA；13genesaboutsignaltransformation；12genesaboutmetabolismandothers8gene，5genesamongthemwereselectedtobeamplificated，andtheexpressionofthemwascoincidentwiththemicroarrayConclusions：Geneexp

6、ressionprofilinginhumangallbladdercarcinomacarlbeanalyzedbycDNAmicroarraysensitively，generously，andeffectivelyTheresultoftheexperimentofmicroarraywasreliableKeywords：GallbladdercarcinomaProfilingeDNAmicroarrayReal—timeRT