血腫抽吸術(shù)治療對實驗性大鼠腦出血后NSE表達的影響.pdf

1、分類號：R364.1密級：不保密UDC：610學(xué)校代碼：11065碩士學(xué)位論文血腫抽吸術(shù)治療對實驗性大鼠腦出血血腫抽吸術(shù)治療對實驗性大鼠腦出血后NSE表達的影響表達的影響李兆星李兆星指導(dǎo)教師王亞男副教授學(xué)科專業(yè)名稱中西醫(yī)結(jié)合臨床論文答辯日期2016年5月29日TheeffectofstereotactichematomaaspirationontheNSEexpressionafterexperimentalintracerebralh

2、emrhageinratAbstractObjectiveToobservetheeffectofstereotactichematomaaspirationontheexpressionofneuronspecificenolase（NSE）aroundhematomactexafterintracerebralhemrhage.MethodsThemodelofintracerebralhemrhagewereestablished

3、byusinginracerebralstereotaxicinjectionoftypeIVcollagenasemethodinmaleadultWistarratswereromlydividedintoshamoperationgroupintracerebralhemrhagegrouphemrhageaspirationgroup.TheneurologicalimpairmentinratswereevaluatedbyB

4、edersonfourgrade.Thebrainwatercontentwasobtainedbywetdryweightmethodat1d2d3d5dafterintracerebralhemrhage.Themphologyofnervecellsinctexwereobservedbythehematoxylineosin（HE）staining.TheexpressionofNSEaroundhematomactexwasd

5、etectedrespectivelybytheimmunohistochemistry（IHC）WesternBlot.ResultsTheexpressionofNSEaroundhematomactexinhematomaaspirationgroupintracerebralhemrhgegroupwassignificantlyhigherthanthatinshamoperationgroupat1d2d3d5dafteri

6、ntracerebralhemrhage（P0.05）buttherewasnosignificantlydifference.ComparedwithintracerebrahemrhagegrouptheexpressionofNSEaroundhematomactexinhemrhageaspirationgroupwassignificantlydecreasedat2d3d5dafterintracerebralhemrhag

7、e（P0.05）.Comparedwithhemrhageaspirationgroupthenumbersofpositivecellsweresignificantlyincreasedthedamageoftheneuronsaroundhematomactexwaswsenedthanthatinintracerebrahemrhagegroup（P0.05）.ConclusionTheexpressionofNSEaround

8、hematomactexafterintracerebralhemrhagewasincreasedobviouslyafterintracerebralhemrhage.HoweverearlyhematomaaspirationcouldweakentheexpressionofNSEreducethenumberofneuronaldegenerationcellsimprovedneurobehavialfunctionsign