灌胃“益氣養(yǎng)陰方”大鼠的血清誘導(dǎo)白血病K562細(xì)胞凋亡的實(shí)驗(yàn)研究.pdf

1、山東中醫(yī)藥大學(xué)碩士學(xué)位論文灌胃“益氣養(yǎng)陰方”大鼠的血清誘導(dǎo)白血病K562細(xì)胞凋亡的實(shí)驗(yàn)研究姓名：臧運(yùn)華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合基礎(chǔ)指導(dǎo)教師：李震2003.4.20ExperimentstudyonserumoftheratsthatAreGivenViQiYangyinformulainducestheApoptosisofHumanLeukemiacelllinesK562Speciality：Basicresearchof

2、integratedTCMandwesternmedicineAuthor：ZangYunhuaTutor：Prof1iZhenAbstractObjectiveToexplorethemechamismoftreatingleukemiawithYiqiYangyinformulaMethodsWehatchedcelllinesK562withhumanleukemiabymeansofserologicalpharmacology

3、Atthesametime，YiqiYangyinformulaisgiventotheratsAndthen，weobserverats’serum’seffectsonmembranefluidity，cellcycleandbcl2geneandmutantp53geneexpressionofcelllinesK562byfluorophotometry，flowcytomeuyandimmunohistochemicResul

4、tsSerumoftheratsgivenYiqiYangyinformulaatlowerdrugconcentrationshaslittleeffectonthemembranefluidityButathigherdrugconcentrationsitcanincreasemembranefluidityofcelllinesK562，thiseffecthasthesametendencytoserumoftheratsgi

5、venarsenicalagentsandthatgivenHUSerumoftheratsgivenYiqiYangyinformulacanincreasemembranefluidityandSphasecells’percentageofcelllinesK562anddecreaseGOGlphasecells’percentage，whichissimilartotheeffectoftherats’serumthatare

6、givenarsenicalagentsSerumoftheratsgivenYiqiYangyinformuladownregulatestheexpressionofbcl2geneandmutantp53gene，andinadditionitinducestheapoptosisofthecelllinesK562ConclusionWithserumoftheratsgivenYiqiYangyinformula，Sphase

7、cellsofcelllinesK562increased，andthenapoptoticcellsincreasedwithadiminutionofSphasecellsThismechanismmayrelatetothefunctionthatincreasemembranefluidityofcelllinesK562anddownregulatetheexpressionofbcI2andmutantp53geneKeyw