應用基因沉默技術培育高油酸大豆.pdf

1、東北師范大學碩士學位論文應用基因沉默技術培育高油酸大豆姓名：宋凱申請學位級別：碩士專業(yè)：植物學指導教師：劉立俠20050501AbstractAsamajorcomponentofplantfattyacid，oleicacidwiththeoxidativestabilityflavorcharacteristicsandphysicalpropertiescontributessignificantlytodecreasetheri

2、skofcardiovasculardiseaseduetotheeffect01“1lesseninglowdensitylipoproteincholesterolinthebloodstreamRecentresearchesshowedthatoleicacidCOuldalsodeclinetheriskofthebreastcancerdevelopmentinwomenThereisagrowingtrendofprodu

3、cinghigholeiccropsusingmoleculartechniquessuchasconventionalselection，inducedmutationandmorerecentlyposttranscriptiona／genesilencingfPTGS)IIIparticularPTGShasbeenusedtodownregulatetheactivityofthedesaturaseenzymesthatcon

4、trolthesynthesisofthemajorseedfattyacidsHowevertheantisenseandcosuppressionstrategiesusedinfattyacidmodificationhavevariableandunpredictableeffectivenessandgenerallyrequiretheselectionfromlargepopulationsoftransgenicplan

5、tstoobtainanacceptablenumberoflinesexhibitingsufficientdegreesoftargetgenesuppressionRecentlyamuchmoreeffectivemethodsofsilencingplantgeneshavebeendevelopedbasedonthediscoverythatPTGSCallbeinvokedatveryhi出frequencyusingt

6、heinvertedrepeatDNAconstructs。SpecificallydesignedgeneticinvertedrepeatsconstructsresultedingenesilencingThisinvertedrepeatsthatencodeRNAhaveregionsofselgcomplementarityandCanreliablygeneratehairpinRNA(bpRNA)transcriptst

7、hatinvokesequencespecificRNAdegradationWehavegeneticallymodifiedthefattyacidcompositionofsoybean(Glycinemax)seedoilusingthetechniqueofhairpinRIgA—medimedgenesilencingBythistechnique，thekeyfattyaciddesatureasegene(gmFAD21

8、1encodingoledyl—phosphatidylcholine(1)一6desatureasewasdown—regulatedresultingintheconsequentlymodificationofthefattyacidcompositionofpeanutoilwithhi曲oleicoilcontentHairpinRNAencodinggeneconstructs(HP)wasdrivenbytheseed—s

9、pecificsoybearllectinpromoterandtrⅫtferedintoSClybeanbyAgrobacteriumtumefacienmediatedmethodtargettingagainstgmFAD2—1Identificationof乜ansgenicplantsbyPCRrevealedthattheconstructshadintegratedintheplantgenomeKeywords：High