魚油對斷奶仔豬抗免疫應激機理研究.pdf

1、中國農業(yè)大學博士學位論文魚油對斷奶仔豬抗免疫應激機理研究姓名：劉玉蘭申請學位級別：博士專業(yè)：動物營養(yǎng)與飼料科學指導教師：李德發(fā)龔利敏20030601AbstractThestudywasconductedtoinvestigatethemechanismoffishoil∞allantimumunologicalagent缸weanlingpigssubjectedto躚ImmunologicalStressTode鋤minetheef

2、fectsofanimmunologicalstress，6weanlingpigs(LargeWhitexLandracexPietrenlwereusedtoestablishinflammatoryparametersandendocrinehormonemeasurementsinthefnstexperimentFourpigswereinjectedipwith200w咖咭BWofEcolilipopolysaccharid

3、e(LPS)，andtwopigswereiⅡjcctedwithanequivalentamountofsterilesalinePlasmasampleswerecollectedat0，1，2and3hpostinjectionforanalysisofinterleukin19qLl口)’prostaglandinE2(PGE9，cortisol，growthhormone(GH)andinsulinlikegrowthfact

4、orI(IGFI)，ILl艮PGEz，cortisol，GHandIGF—IWCl“e丑llaffectedbyLPStreaunentTheLPSchallengeincreased1Llp，cortisolandPOE2妒005)andwhileGHandIGFIbothdecreasedcPO05)Tofurtherevaluatetheefficacyoffishon觴髓antiixmonnologicalagent72wean

5、lingpigswereusedtoinvestigatetheeffectsoffishoilsupplementationongrowmperformance，int3attLmatoryparametersandendocrinehormonesfollowingEcoliLPSchallengeina2x2factorialdesignThefactorsincludeddietaryfattype(7％cornoilor7％f

6、ishoil)andimmuaolo#calchallenge皿PSorsaline)Ond14and21pigsWeleinjcotedipWimeither200址g／kgBWofLPSorsterilesalineBloodsampleswerecollectedforanalysisofplasmaIL一1B，POE2，cortisol，GHandIGFIat3hpostinjectionandat2dpostinjection

7、for／n協陽analysisofbloodlymphocytproliferation，IL一1pandinterleukin2aL2)productionFishoilimprovedADGandADFI(P010)，attenuatedtheelevationofILl口妒(005)，PGE2(P(0001)andcortisol儼(0001)andthedecreaseofIGF(P(O，01)a腦LPSchallengeFjs

8、h0ilsupplementationinhibitedlymphocyteproliferationⅡ，1pandIL2production儼010)Theresultssuggestthatfishoilactsasanantiimmunologicalagentwhichmaybeassociatedwithdownregnlatiunoftheactivatedililnnmesystemespeciallyproinflamm

9、atorycytokinesInordertodetermineiftheEPAandDHAinfishoilproducedtheprimaryilnlnuneinhibitoryeffectpcr／phoralbloodlymphocytesofweanlingpigswereincubatedwithvariousconcenWations(0，20，40，60，80，100pg／mL)ofeicosapentaenoicacid

10、(EPA)anddocosahexaenoicacidfDRA)，withlinoleicacid(LA)asconU01EPA，DHAend11ighlevelsofLApredominantlysuppressed也一1B(，《O05)and152滬O，05)productionandsubsequentlymphocyteproliferation‘P005)ComparedwithLA，EPAorDHAresultedinast

11、rongerinhibitionoftheseparametersTheseresultssuggestthatfishoilli】【elyexertsitseffects∞anantiimmunologicalagentthroughtheactionofEPAandDHAInordertoelucidatethemechanismsbywhichfishoilexerteditseffects∞anantiimmunological

12、agentpenpheralbloodlymphocytesofweanlingpigsWOleincubatedwithEPA，DHAorLAtoinvestigateseveralintraceUularsignalingmoleculesEPAandDHAinhibitedlymphocyteproliferationendcytokineproductionduetoinductionofnitricoxide，cAMPprod

13、uctionandinhibitionofproteinkinaseCactivityTheseresultssuggestfishoilactsasartanti，immunologicalagentduetoalterationofintracdlularsignaltransductionKeywords：Weanlingpigs，Immunologicalstress，Fishoil，llnnlunefunction，Signa