生姜原生質體培養(yǎng)與體細胞變異.pdf

1、Y773403分類譬霄級單位代碼：10019學號：B02066‘專峨巖嚏夭等博士學位論文生姜原生質體培養(yǎng)與體細胞變異ProtoplastCultureandSomaticVariationofGinger(ZingiberofficinaleRosc)(國家科技攻關資助項日60|1002)(農業(yè)部農業(yè)結構調整項目040602B)研究生：酆甚垡指導教師：一毯拯豎熬援中請學位門類級別：盛堂埴專業(yè)名稱：蘊苤堂研冤方向：益苤生理生盆工生塑堂所存

2、學院：丞堂生生物整盔堂逮二零零五年五月中國農業(yè)大學博士論文摘受AbstractGinger(Zingiberofficma缸Rose)isacultivarproducingrhizomesofthefamilyZingiberaceaeItismostlydistributedintheAsiantropicalandsubtropicsregionAndSouthAfrica，NorthAmericaandAustraliaalso

3、plantgingersGingersarerichinvolatileoilsandwidelyusedasacondiments，dyes，beverage，medicineandvegetableGingersannuallypropagatethemselvesbyrhizomeswithlowproliferationrateTheyareheavilyattackedbysoilboropathogensAndthequal

4、ityofrhizomesdegeneratesforthelongtermagamicpropagationGingersseldomflowersandhaveseeds，soitisdifficulttocultivatenewvarietythroughcrossbreedingInthispaperinvitrotissueculturetechniqueswerecarriedoutincludingsomaticandem

5、byogeniccallusinductionembryogenicsusppeosioncultureandprotoplastculture1Somaticembryogcniccalliof4gingergenotypeswereproducedSomaticembryogeniccalluswasinducedfromgingershoottipsonMSmediumsupplementedwith10mg／L2，4Dand02

6、mgU1KTThecallus(about1Scmindiameter)weretransformedintotheMS02mg‘L‘24一D50mgL1BA3％socrose07％ag礬andshootsandrootswereformedAndshootsdevelopedintocompleteplantletswithrhizomesonsolidMSmediumsupplementedwith30ragL1BAand01mgo

7、L1NAAand6％sucroseTheeffectofphytohormonesonembryogeniccallusformationandredifferenciationwasdiscussedTheeffectofsucroseonrhizomeformationwasresearched2Somaticembryogenicsuspensionculmresof4gingergenotypcswere：established

8、Fromwhich，regeneratedplantletswereobtainedTherelationshipbetweenthedryweightfDw)ofsuspensionculturesandpHchangesinmediumwasalsodiscussedTheeffectsofinoculationandAgN03Oilthegrowthofembryogenlcsuspensionswerealsodiscussed

9、TheoptimumconcentrationofinoculationandlevelofAgN03were10％and60mgL～respectively3Protoplastswereisolatedfromembryogenicsuspensionsincubatinginenzymesolutioncontaining40％celluloseOnozukaR10，10％maceroymeR10，01％PectolyaseY23

10、，05％CaCl2，01％IVIESand11％mannit01Theenzymemixturewerekeptinastationarypositionfor6handshaken(60rpm)for6hat27℃Theyieldandviabilitywere627X1069—and907％respectivelyPurifyingprotoplastswereculturedinitiallyinshallowliquidlaye

11、rs4Thechromosomenumbersofembryogeniccallusderivedplants，embyogenicsuspension—derivedplantsandprotoplastderivedplantsWere22，equaltotheirparentplantsTherewemdifferencesofmorphologicalcharactersbetweeninvitroplantsandpsxcnt

12、plantssuch鵲leafthick，leafcolorandLeafindexTheRAPDanalysisofregeneratedplantsindicatedrelativelysomaticvariationThechromosomenumberdeterminationandRAPDanalysisofembyogeniccallusandembryoenicsuspensionderivedandprotoplastd