18784.基于天然化學(xué)連接的fret巰基探針研究

1、南開大學(xué)碩士學(xué)位論文基于天然化學(xué)連接的FRET巰基探針研究姓名：鄭萌申請學(xué)位級別：碩士專業(yè)：化學(xué)生物學(xué)指導(dǎo)教師：席真201205Abstract_____●——__________——_______—●———___________———__———____________—__—________——_一一________●—_—___________—●__—_________一AbstractSincethiolsplaycrucial

2、rolesinlifesystem，fluctuationinthelevelofcellularmercaptomoleculeshasgreattodowithvariousdiseasesTheconcentrationofcysctein(Cys)orhomocystein(Hey)isthebiomarkerofmanydiseases，whiletheglutathione，whichistherichestintracel

3、lular：non—proteinthiol，takestheresponsibilityofdiversityofcellularactivities，includingmaintenanceofintracellularreduction，xenobioticmetabolism，intracellularsignaltransductionandgeneregulationFluorescencespectroscopyisani

4、mportantwaytodetectthiolsinorganismsduetoitshighsensitivitygooddynamicrange，simplicityandlowdetectionlimitBesides，thegreatestadvantageot、fluorescentprobewouldbeintracellulardetectionFluorescentResonanceEnergyTransfer(FRE

5、’F)hasbeenwidelyusedinchemistryandlifesciencesinceitwasdiscoverednearly60yearsagoInthepastfewyears，thedesign，synthesisandcharacterizationofFRETfluorescentprobeforthiolsbycleavageofdisulfidebondbymercaptohasalreadybeenrep

6、ortedHoweverwedesignandsynthesizeaFRETfluorescentprobeforthiolsaccordingtothemechanismofnativechemicalligation(NCL)，whichsimulatestheproteinsplicingprocessAfterthetwopartsofaFRETpairareboundtobothsidesofthioesterthiolest

7、ercleavagewithmercaptocompoundwouldbreaktheFRETsystem，SOthatthealternationoffluorescentsignalcouldbedetectedThe：probeemployshighFRETefficiencyconsiderableselectivityanddifferentrecognitiontowardsstructurallydiversifiedme