25265.cawrky5啟動子分離及其在煙草瞬間表達系統(tǒng)中分析

1、福建農(nóng)林大學碩士學位論文CaWRKY5啟動子分離及其在煙草瞬間表達系統(tǒng)中分析姓名：劉志欽申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：何水林20100401福建農(nóng)林大學2010屆碩士畢業(yè)論文IsolationandExpressionAnalysisofthePromoterofCaWRKY5inTbaccoTransientExISyst‘0acco1ranslentxDresslonsystemlnlAbstractProt

2、einsinWRKYsuperfamilyincludemanyplantspecifictranscriptionfactors，whichplayimportantrolesintheregulationofplantgrowth，developmentandstressesresponsesCaWRKY5isamemberofthepepper(CapsicumannuumL)WRKYfamilyOurpreviousstudyf

3、oundthattheexpressionofCaWRKY5wasinducedbyheat，highsalinityandpathogeninfectionindicatingthepossibleinvolvementofCaWRKY5inplantdefensereactionagainstbioticandabioticstressesHoweverthemolecularmechanismofthetranscriptione

4、xpressionofCaWRKY5againstdifferentstressesremainsunclearInthisstudytogaininsightintothemolecularbasisandpossiblesignalingpathwayoftheinductionofCaWRKY5responsetoenvironmentstress，weisolatedthepromoterofCaWRKY5Deletionana

5、lysisoftheCaWRKY5promoteragainstdifferentstressesaswellasexogenoushormoneswascarriedoutviaanAgrobacterium—mediatedtransientexpressionassayintobaccoleavesTheresultsareasfollowings：(1)The936bpupstreampromotersequenceoftheC

6、aWRKY5wasisolatedfromthegenomicDNAofpepperbyLA—PCRBioinformaticsanalysisshowedthattheTATA—boxofthepromoterlocatesin一140bpto136bp，andseveralc／s—actingelementsincludingWbox，Sbox，SAresponsiveelement，ABREresponsivetodifferen

7、tstressesorhormoneswerefoundinthepromoter(2)The936bpregionupstreamoftranslationstartcodoninCaWRKY5seemedtobeanintactpromoterThefragmentfromtranslationStartcodonto936bpandfromtranslationstartcodonto一886bpinthepromoterwere

8、enoughforGUSexpressionagainstpathogeninfectionandMeJAinduction，Howeverthefragmentfromtranslationstartcodonto489bpabolishedtheresponseofGUStopathogeninfectionandMeJAinductionTheseresultssuggestedthatwithinregionfrom886bpt